The University of Tennessee Health Science Center

• Ph.D., University of Akron, Akron, Ohio, Analytical chemistry
• M.S., Prague Institute of Chemical Technology, Prague, Czechoslovakia, Organic chemistry

 bioanalytical chemistry; mass spectrometry; proteomics; phosphoproteomics

Bioanalytical chemistry; biological mass spectrometry; qualitative and quantitative proteomics; targeted protein and phosphoprotein identification/characterization.

• Proteomics of the retina in health and disease
• Phosphoproteomics of the mammalian pituitary
• High-sensitivity phosphoprotein characterization
• Design and development of new and improved analytical platforms for proteomics.

1. Iannaccone, A, Hollingsworth, TJ, Koirala, D, New, DD, Lenchik, NI, Beranova-Giorgianni, S, Gerling, IC, Radic, MZ, Giorgianni, F. Retinal pigment epithelium and microglia express the CD5 antigen-like protein, a novel autoantigen in age-related macular degeneration. Exp Eye Res, 155, 64-74, 2017.
2. Giorgianni, F, Beranova-Giorgianni, S. Phosphoproteome Discovery in Human Biological Fluids. Proteomes, 4 (4), 2016.
3. Iannaccone, A, Giorgianni, F, New, DD, Hollingsworth, TJ, Umfress, A, Alhatem, AH, Neeli, I, Lenchik, NI, Jennings, BJ, Calzada, JI, Satterfield, S, Mathews, D, Diaz, RI, Harris, T, Johnson, KC, Charles, S, Kritchevsky, SB, Gerling, IC, Beranova-Giorgianni, S, Radic, MZ, ,. Circulating Autoantibodies in Age-Related Macular Degeneration Recognize Human Macular Tissue Antigens Implicated in Autophagy, Immunomodulation, and Protection from Oxidative Stress and Apoptosis. PLoS One, 10 (12), e0145323, 2015.
4. Dong, Q, Giorgianni, F, Beranova-Giorgianni, S, Deng, X, O'Meally, RN, Bridges, D, Park, EA, Cole, RN, Elam, MB, Raghow, R. Glycogen synthase kinase-3-mediated phosphorylation of serine 73 targets sterol response element binding protein-1c (SREBP-1c) for proteasomal degradation. Biosci Rep, 36 (1), e00284, 2015.
5. Giorgianni, F, Koirala, D, Beranova-Giorgianni, S. Proteomics of the human pituitary tissue: bioanalytical methods and applications. Bioanalysis, 6 (14), 1989-2003, 2014.
6. Dong, Q, Giorgianni, F, Deng, X, Beranova-Giorgianni, S, Bridges, D, Park, EA, Raghow, R, Elam, MB. Phosphorylation of sterol regulatory element binding protein-1a by protein kinase A (PKA) regulates transcriptional activity. Biochem Biophys Res Commun, 449 (4), 449-54, 2014.
7. Giorgianni, F, Koirala, D, Weber, KT, Beranova-Giorgianni, S. Proteome analysis of subsarcolemmal cardiomyocyte mitochondria: a comparison of different analytical platforms. Int J Mol Sci, 15 (6), 9285-301, 2014.
8. Giorgianni, F, Usman Khan, M, Weber, KT, Gerling, IC, Beranova-Giorgianni, S. Phosphoproteome mapping of cardiomyocyte mitochondria in a rat model of heart failure. Mol Cell Biochem, 389 (1-2), 159-67, 2014.
9. Giorgianni, F, Mileo, V, Desiderio, DM, Catinella, S, Beranova-Giorgianni, S. Characterization of the phosphoproteome in human bronchoalveolar lavage fluid. Int J Proteomics, 2012, 460261, 2012.
10. Chen, L, Fang, B, Giorgianni, F, Gingrich, JR, Beranova-Giorgianni, S. Investigation of phosphoprotein signatures of archived prostate cancer tissue specimens via proteomic analysis. Electrophoresis, 32 (15), 1984-91, 2011.
11. Bolen, AL, Naren, AP, Yarlagadda, S, Beranova-Giorgianni, S, Chen, L, Norman, D, Baker, DL, Rowland, MM, Best, MD, Sano, T, Tsukahara, T, Liliom, K, Igarashi, Y, Tigyi, G. The phospholipase A1 activity of lysophospholipase A-I links platelet activation to LPA production during blood coagulation. J Lipid Res, 52 (5), 958-70, 2011.
12. Wang, X, Tong, Y, Giorgianni, F, Beranova-Giorgianni, S, Penn, JS, Jablonski, MM. Cellular retinol binding protein 1 modulates photoreceptor outer segment folding in the isolated eye. Dev Neurobiol, 70 (9), 623-35, 2010.
13. Nookala, S, Gandrakota, R, Wohabrebbi, A, Wang, X, Howell, D, Giorgianni, F, Beranova-Giorgianni, S, Desiderio, DM, Jablonski, MM. In search of the identity of the XAP-1 antigen: a protein localized to cone outer segments. Invest Ophthalmol Vis Sci, 51 (5), 2736-43, 2010.
14. Chen, L, Giorgianni, F, Beranova-Giorgianni, S. Characterization of the phosphoproteome in LNCaP prostate cancer cells by in-gel isoelectric focusing and tandem mass spectrometry. J Proteome Res, 9 (1), 174-8, 2010.
15. Beranova-Giorgianni, S, Desiderio, DM, Giorgianni, F. Phosphoproteome analysis by in-gel isoelectric focusing and tandem mass spectrometry. Methods Mol Biol, 519, 383-96, 2009.
16. Wang, X, Nookala, S, Narayanan, C, Giorgianni, F, Beranova-Giorgianni, S, McCollum, G, Gerling, I, Penn, JS, Jablonski, MM. Proteomic analysis of the retina: removal of RPE alters outer segment assembly and retinal protein expression. Glia, 57 (4), 380-92, 2009.
17. Pabst, MJ, Pabst, KM, Handsman, DB, Beranova-Giorgianni, S, Giorgianni, F. Proteome of monocyte priming by lipopolysaccharide, including changes in interleukin-1beta and leukocyte elastase inhibitor. Proteome Sci, 6, 13, 2007.
18. Giorgianni, F, Zhao, Y, Desiderio, DM, Beranova-Giorgianni, S. Toward a global characterization of the phosphoproteome in prostate cancer cells: identification of phosphoproteins in the LNCaP cell line. Electrophoresis, 28 (12), 2027-34, 2007.
19. Beranova-Giorgianni, S, Zhao, Y, Desiderio, DM, Giorgianni, F. Phosphoproteomic analysis of the human pituitary. Pituitary, 9 (2), 109-20, 2006.
20. Zhao, Y, Giorgianni, F, Desiderio, DM, Fang, B, Beranova-Giorgianni, S. Toward a global analysis of the human pituitary proteome by multiple gel-based technology. Anal Chem, 77 (16), 5324-31, 2005.
21. Giorgianni, F, Cappiello, A, Beranova-Giorgianni, S, Palma, P, Trufelli, H, Desiderio, DM. LC-MS/MS analysis of peptides with methanol as organic modifier: improved limits of detection. Anal Chem, 76 (23), 7028-38, 2004.
22. Giorgianni, F, Beranova-Giorgianni, S, Desiderio, DM. Identification and characterization of phosphorylated proteins in the human pituitary. Proteomics, 4 (3), 587-98, 2004.
23. Gadgil, HS, Pabst, KM, Giorgianni, F, Umstot, ES, Desiderio, DM, Beranova-Giorgianni, S, Gerling, IC, Pabst, MJ. Proteome of monocytes primed with lipopolysaccharide: analysis of the abundant proteins. Proteomics, 3 (9), 1767-80, 2003.
24. Giorgianni, F, Desiderio, DM, Beranova-Giorgianni, S. Proteome analysis using isoelectric focusing in immobilized pH gradient gels followed by mass spectrometry. Electrophoresis, 24 (1-2), 253-9, 2003.
25. Beranova-Giorgianni, S, Giorgianni, F, Desiderio, DM. Analysis of the proteome in the human pituitary. Proteomics, 2 (5), 534-42, 2002.