SHUKLA, PRADEEP KUMAR

Assistant Professor
Physiology

Office: C317 VAN VLEET CANCER CENTER
3 NORTH DUNLAP STREET
MEMPHIS TN 381632113
Tel: (901) 448-3019
pshukla2@uthsc.edu

Education

  • Staff fellow, NIH/NINDS, Bethesda, MD, Neuroscience
  • Research Associate, Northwestern University Chicago, IL, Neuroscience
  • Postdoctoral fellow, University of Illinois at Chicago, IL, Neuroscience
  • Ph.D., IITR Lucknow/Dr. RML Avadh University, India, Biochemistry
  • M.S., Dr. RML Avadh University, India, Biochemistry

Research Description

Major projects

1) Alcohol‐induced gut barrier dysfunction and its prevention. 

2) The effect of ethanol on azoxymethane and dextran sulfate sodium (AOM+DSS)‐induced colonic tumorigenesis. 

3) Radiation‐induced barrier dysfunction and its mitigation by various pharmacological agents.

Research Keywords

 Alcohol, Fetal Alcohol Spectrum Disorder, Ionization Radiation, Oxidative Stress, Gut Brain Axis, Epigenetics, Chromatin Remodeling

Research Interest/Specialty

Alcohol''s Effects on Gut and Brain

Publications

  1. Bhandari, S, Shukla, P, Almabadi, H, Sahay, P, Rao, R, Pradhan, P. Optical study of stress hormone-induced nanoscale structural alteration in brain using partial wave spectroscopic (PWS) microscopy. J Biophotonics, e201800002, 2018.
  2. Meena, AS, Shukla, PK, Sheth, P, Rao, R. EGF receptor plays a role in the mechanism of glutamine-mediated prevention of alcohol-induced gut barrier dysfunction and liver injury. J Nutr Biochem, 64, 128-143, 2018.
  3. Shukla, PK, Meena, AS, Rao, V, Rao, RG, Balazs, L, Rao, R. Human Defensin-5 Blocks Ethanol and Colitis-Induced Dysbiosis, Tight Junction Disruption and Inflammation in Mouse Intestine. Sci Rep, 8 (1), 16241, 2018.
  4. Thompson, KE, Ray, RM, Alli, S, Ge, W, Boler, A, Shannon McCool, W, Meena, AS, Shukla, PK, Rao, R, Johnson, LR, Miller, MA, Tigyi, GJ. Prevention and treatment of secretory diarrhea by the lysophosphatidic acid analog Rx100. Exp Biol Med (Maywood), 1535370218803349, 2018.
  5. Shukla, PK, Meena, AS, Manda, B, Gomes-Solecki, M, Dietrich, P, Dragatsis, I, Rao, R. Lactobacillus plantarum prevents and mitigates alcohol-induced disruption of colonic epithelial tight junctions, endotoxemia, and liver damage by an EGF receptor-dependent mechanism. FASEB J, fj201800351R, 2018.
  6. Shukla, PK, Meena, AS, Rao, R, Rao, R. Deletion of TLR-4 attenuates fetal alcohol exposure-induced gene expression and social interaction deficits. Alcohol, 73, 73-78, 2018.
  7. Manda, B, Mir, H, Gangwar, R, Meena, AS, Amin, S, Shukla, PK, Dalal, K, Suzuki, T, Rao, RK. Phosphorylation hotspot in the C-terminal domain of occludin regulates the dynamics of epithelial junctional complexes. J Cell Sci, 2018.
  8. Boule, LA, Ju, C, Agudelo, M, Parira, T, Cannon, A, Davis, B, Eby, J, Cresci, G, Samuelson, DR, Shukla, P, Alrefai, WA, Sureshchandra, S, Pandey, SC, Schnabl, B, Curtis, BJ, Wyatt, TA, Choudhry, MA, Kovacs, EJ. Summary of the 2016 Alcohol and Immunology Research Interest Group (AIRIG) meeting. Alcohol, 66, 35-43, 2018.
  9. Sahay, P, Shukla, PK, Ghimire, HM, Almabadi, HM, Tripathi, V, Mohanty, SK, Rao, R, Pradhan, P. Quantitative analysis of nanoscale intranuclear structural alterations in hippocampal cells in chronic alcoholism via transmission electron microscopy imaging. Phys Biol, 14 (2), 026001, 2017.
  10. Gangwar, R, Meena, AS, Shukla, PK, Nagaraja, AS, Dorniak, PL, Pallikuth, S, Waters, CM, Sood, A, Rao, R. Calcium-mediated oxidative stress: a common mechanism in tight junction disruption by different types of cellular stress. Biochem J, 474 (5), 731-749, 2017.
  11. Samak, G, Gangwar, R, Meena, AS, Rao, RG, Shukla, PK, Manda, B, Narayanan, D, Jaggar, JH, Rao, R. Calcium Channels and Oxidative Stress Mediate a Synergistic Disruption of Tight Junctions by Ethanol and Acetaldehyde in Caco-2 Cell Monolayers. Sci Rep, 6, 38899, 2016.
  12. Shukla, PK, Gangwar, R, Manda, B, Meena, AS, Yadav, N, Szabo, E, Balogh, A, Lee, SC, Tigyi, G, Rao, R. Rapid disruption of intestinal epithelial tight junction and barrier dysfunction by ionizing radiation in mouse colon in vivo: protection by N-acetyl-l-cysteine. Am J Physiol Gastrointest Liver Physiol, 310 (9), G705-15, 2016.
  13. Mir, H, Meena, AS, Chaudhry, KK, Shukla, PK, Gangwar, R, Manda, B, Padala, MK, Shen, L, Turner, JR, Dietrich, P, Dragatsis, I, Rao, R. Occludin deficiency promotes ethanol-induced disruption of colonic epithelial junctions, gut barrier dysfunction and liver damage in mice. Biochim Biophys Acta, 1860 (4), 765-74, 2016.
  14. Chaudhry, KK, Shukla, PK, Mir, H, Manda, B, Gangwar, R, Yadav, N, McMullen, M, Nagy, LE, Rao, R. Glutamine supplementation attenuates ethanol-induced disruption of apical junctional complexes in colonic epithelium and ameliorates gut barrier dysfunction and fatty liver in mice. J Nutr Biochem, 27, 16-26, 2016.
  15. Balogh, A, Shimizu, Y, Lee, SC, Norman, DD, Gangwar, R, Bavaria, M, Moon, C, Shukla, P, Rao, R, Ray, R, Naren, AP, Banerjee, S, Banerje, S, Miller, DD, Balazs, L, Pelus, L, Tigyi, G. The autotaxin-LPA2 GPCR axis is modulated by γ-irradiation and facilitates DNA damage repair. Cell Signal, 27 (9), 1751-62, 2015.
  16. Shukla, PK, Chaudhry, KK, Mir, H, Gangwar, R, Yadav, N, Manda, B, Meena, AS, Rao, R. Chronic ethanol feeding promotes azoxymethane and dextran sulfate sodium-induced colonic tumorigenesis potentially by enhancing mucosal inflammation. BMC Cancer, 16 (1), 189, 2015.
  17. Chaudhry, KK, Samak, G, Shukla, PK, Mir, H, Gangwar, R, Manda, B, Isse, T, Kawamoto, T, Salaspuro, M, Kaihovaara, P, Dietrich, P, Dragatsis, I, Nagy, LE, Rao, RK. ALDH2 Deficiency Promotes Ethanol-Induced Gut Barrier Dysfunction and Fatty Liver in Mice. Alcohol Clin Exp Res, 39 (8), 1465-75, 2015.
  18. Tunc-Ozcan, E, Ullmann, TM, Shukla, PK, Redei, EE. Low-dose thyroxine attenuates autism-associated adverse effects of fetal alcohol in male offspring's social behavior and hippocampal gene expression. Alcohol Clin Exp Res, 37 (11), 1986-95, 2013.
  19. Kotagale, NR, Shirbhate, SH, Shukla, P, Ugale, RR. Agmatine attenuates neuropathic pain in sciatic nerve ligated rats: modulation by hippocampal sigma receptors. Eur J Pharmacol, 714 (1-3), 424-31, 2013.
  20. Sittig, LJ, Herzing, LB, Xie, H, Batra, KK, Shukla, PK, Redei, EE. Excess folate during adolescence suppresses thyroid function with permanent deficits in motivation and spatial memory. Genes Brain Behav, 11 (2), 193-200, 2012.
  21. Andrus, BM, Blizinsky, K, Vedell, PT, Dennis, K, Shukla, PK, Schaffer, DJ, Radulovic, J, Churchill, GA, Redei, EE. Gene expression patterns in the hippocampus and amygdala of endogenous depression and chronic stress models. Mol Psychiatry, 17 (1), 49-61, 2012.
  22. Yadav, RS, Chandravanshi, LP, Shukla, RK, Sankhwar, ML, Ansari, RW, Shukla, PK, Pant, AB, Khanna, VK. Neuroprotective efficacy of curcumin in arsenic induced cholinergic dysfunctions in rats. Neurotoxicology, 32 (6), 760-8, 2011.
  23. Sittig, LJ, Herzing, LB, Shukla, PK, Redei, EE. Parent-of-origin allelic contributions to deiodinase-3 expression elicit localized hyperthyroid milieu in the hippocampus. Mol Psychiatry, 16 (8), 786-7, 2011.
  24. Sittig, LJ, Shukla, PK, Herzing, LB, Redei, EE. Strain-specific vulnerability to alcohol exposure in utero via hippocampal parent-of-origin expression of deiodinase-III. FASEB J, 25 (7), 2313-24, 2011.
  25. Shukla, PK, Sittig, LJ, Ullmann, TM, Redei, EE. Candidate placental biomarkers for intrauterine alcohol exposure. Alcohol Clin Exp Res, 35 (3), 559-65, 2011.
  26. Schaffer, DJ, Tunc-Ozcan, E, Shukla, PK, Volenec, A, Redei, EE. Nuclear orphan receptor Nor-1 contributes to depressive behavior in the Wistar-Kyoto rat model of depression. Brain Res, 1362, 32-9, 2010.
  27. Shukla, PK, Sittig, LJ, Andrus, BM, Schaffer, DJ, Batra, KK, Redei, EE. Prenatal thyroxine treatment disparately affects peripheral and amygdala thyroid hormone levels. Psychoneuroendocrinology, 35 (6), 791-7, 2010.
  28. Shukla, PK, Khanna, VK, Ali, MM, Khan, MY, Srimal, RC. Anti-ischemic effect of curcumin in rat brain. Neurochem Res, 33 (6), 1036-43, 2008.
  29. Luo, F, Yang, C, Chen, Y, Shukla, P, Tang, L, Wang, LX, Wang, ZJ. Reversal of chronic inflammatory pain by acute inhibition of Ca2+/calmodulin-dependent protein kinase II. J Pharmacol Exp Ther, 325 (1), 267-75, 2008.
  30. Tang, L, Shukla, PK, Wang, ZJ. Disruption of acute opioid dependence by antisense oligodeoxynucleotides targeting neurogranin. Brain Res, 1143, 78-82, 2007.
  31. Shukla, PK, Tang, L, Wang, ZJ. Phosphorylation of neurogranin, protein kinase C, and Ca2+/calmodulin dependent protein kinase II in opioid tolerance and dependence. Neurosci Lett, 404 (3), 266-9, 2006.
  32. Tang, L, Shukla, PK, Wang, ZJ. Attenuation of opioid tolerance by antisense oligodeoxynucleotides targeting neurogranin. Eur J Pharmacol, 542 (1-3), 106-7, 2006.
  33. Tang, L, Shukla, PK, Wang, LX, Wang, ZJ. Reversal of morphine antinociceptive tolerance and dependence by the acute supraspinal inhibition of Ca(2+)/calmodulin-dependent protein kinase II. J Pharmacol Exp Ther, 317 (2), 901-9, 2006.
  34. Shukla, PK, Khanna, VK, Ali, MM, Maurya, R, Khan, MY, Srimal, RC. Neuroprotective effect of Acorus calamus against middle cerebral artery occlusion-induced ischaemia in rat. Hum Exp Toxicol, 25 (4), 187-94, 2006.
  35. Tang, L, Shukla, PK, Wang, ZJ. Trifluoperazine, an orally available clinically used drug, disrupts opioid antinociceptive tolerance. Neurosci Lett, 397 (1-2), 1-4, 2005.
  36. Pandey, GN, Dwivedi, Y, Ren, X, Rizavi, HS, Mondal, AC, Shukla, PK, Conley, RR. Brain region specific alterations in the protein and mRNA levels of protein kinase A subunits in the post-mortem brain of teenage suicide victims. Neuropsychopharmacology, 30 (8), 1548-56, 2005.
  37. Dwivedi, Y, Mondal, AC, Rizavi, HS, Shukla, PK, Pandey, GN. Single and repeated stress-induced modulation of phospholipase C catalytic activity and expression: role in LH behavior. Neuropsychopharmacology, 30 (3), 473-83, 2005.
  38. Dwivedi, Y, Mondal, AC, Shukla, PK, Rizavi, HS, Lyons, J. Altered protein kinase a in brain of learned helpless rats: effects of acute and repeated stress. Biol Psychiatry, 56 (1), 30-40, 2004.
  39. Dwivedi, Y, Rizavi, HS, Shukla, PK, Lyons, J, Faludi, G, Palkovits, M, Sarosi, A, Conley, RR, Roberts, RC, Tamminga, CA, Pandey, GN. Protein kinase A in postmortem brain of depressed suicide victims: altered expression of specific regulatory and catalytic subunits. Biol Psychiatry, 55 (3), 234-43, 2004.
  40. Shukla, PK, Khanna, VK, Khan, MY, Srimal, RC. Protective effect of curcumin against lead neurotoxicity in rat. Hum Exp Toxicol, 22 (12), 653-8, 2003.
  41. Shukla, PK, Khanna, VK, Ali, MM, Maurya, RR, Handa, SS, Srimal, RC. Protective effect of acorus calamus against acrylamide induced neurotoxicity. Phytother Res, 16 (3), 256-60, 2002.