Nikhlesh Singh, DVM, PhD

Assistant Professor
Physiology

Office: 426 NASH RESEARCH BUILDING
894 UNION AVENUE
MEMPHIS TN 381630000
Tel: (901) 448-1788
nsingh2@uthsc.edu

Education

  • PostDoc, University of Tennessee Health Science Center, Cardiovascular Biology
  • Ph.D., NDRI, India, Animal Biochemistry
  • Master of Veterinary Science, NDRI, India, Animal Biochemistry
  • D.V.M (B.V.Sc & A.H.), GB Pant University of Agri. & Tech., India, Veterinary Medicine

Research Interest

1) Atherosclerosis

2) Restenosis

3) Retinopathy of Prematurity

4) Angiogenesis

5) Colon Carcinogenesis

Publications

  1. Raghavan, S, Singh, NK, Gali, S, Mani, AM, Rao, GN. Protein Kinase Cθ Via Activating Transcription Factor 2-Mediated CD36 Expression and Foam Cell Formation of Ly6C Cells Contributes to Atherosclerosis. Circulation, 138 (21), 2395-2412, 2018.
  2. Singh, NK, Rao, GN. Emerging role of 12/15-Lipoxygenase (ALOX15) in human pathologies. Prog Lipid Res, 73, 28-45, 2018.
  3. Mani, AM, Chattopadhyay, R, Singh, NK, Rao, GN. Cholesterol crystals increase vascular permeability by inactivating SHP2 and disrupting adherens junctions. Free Radic Biol Med, 123, 72-84, 2018.
  4. Raghavan, S, Singh, NK, Mani, AM, Rao, GN. Protease-activated receptor 1 inhibits cholesterol efflux and promotes atherogenesis via cullin 3-mediated degradation of the ABCA1 transporter. J Biol Chem, 293 (27), 10574-10589, 2018.
  5. Chattopadhyay, R, Mani, AM, Singh, NK, Rao, GN. Resolvin D1 blocks HO-mediated inhibitory crosstalk between SHP2 and PP2A and suppresses endothelial-monocyte interactions. Free Radic Biol Med, 117, 119-131, 2018.
  6. Janjanam, J, Zhang, B, Mani, AM, Singh, NK, Traylor, JG, Orr, AW, Rao, GN. LIM and cysteine-rich domains 1 is required for thrombin-induced smooth muscle cell proliferation and promotes atherogenesis. J Biol Chem, 293 (9), 3088-3103, 2018.
  7. Kotla S, Singh NK, Rao GN.. ROS via BTK-p300-STAT1-PPARγ signaling activation mediates cholesterol crystals-induced CD36 expression and foam cell formation.. Redox Biol. (11), 350-364, 2017.
  8. Singh NK, Janjanam J, Rao GN.. p115 RhoGEF activates the Rac1 GTPase signaling cascade in MCP1 chemokine-induced vascular smooth muscle cell migration and proliferation.. J Biol Chem., 34 (292), 14080-91, 2017.
  9. Kotla S, Singh NK, Kirchhofer D, Rao GN.. Heterodimers of the transcriptional factors NFATc3 and FosB mediate tissue factor expression for 15(S)-hydroxyeicosatetraenoic acid-induced monocyte trafficking.. J Biol Chem., 36 (292), 14885-01, 2017.
  10. Kumar, R, Janjanam, J, Singh, NK, Rao, GN. A new role for cofilin in retinal neovascularization. J Cell Sci, 129 (6), 1234-49, 2016.
  11. Kumar R, Singh NK, Rao GN.. Proline-rich tyrosine kinase 2 via enhancing signal transducer and activator of transcription 3-dependent cJun expression mediates retinal neovascularization.. Sci Rep. (6), 26480, 2016.
  12. Chattopadhyay, R, Tinnikov, A, Dyukova, E, Singh, NK, Kotla, S, Mobley, JA, Rao, GN. 12/15-Lipoxygenase-dependent ROS production is required for diet-induced endothelial barrier dysfunction. J Lipid Res, 56 (3), 562-77, 2015.
  13. Singh NK, Kotla S, Kumar R, Rao GN.. Cyclic AMP Response Element Binding Protein Mediates Pathological Retinal Neovascularization via Modulating DLL4-NOTCH1 Signaling.. EBioMedicine, 2 (11), 1767-1784, 2015.
  14. Kotla, S, Singh, NK, Traylor, JG, Orr, AW, Rao, GN. ROS-dependent Syk and Pyk2-mediated STAT1 activation is required for 15(S)-hydroxyeicosatetraenoic acid-induced CD36 expression and foam cell formation. Free Radic Biol Med, 76, 147-62, 2014.
  15. Singh, NK, Kotla, S, Dyukova, E, Traylor, JG, Orr, AW, Chernoff, J, Marion, TN, Rao, GN. Disruption of p21-activated kinase 1 gene diminishes atherosclerosis in apolipoprotein E-deficient mice. Nat Commun, 6, 7450, 2014.
  16. Chattopadhyay, R, Dyukova, E, Singh, NK, Ohba, M, Mobley, JA, Rao, GN. Vascular endothelial tight junctions and barrier function are disrupted by 15(S)-hydroxyeicosatetraenoic acid partly via protein kinase C ε-mediated zona occludens-1 phosphorylation at threonine 770/772. J Biol Chem, 289 (6), 3148-63, 2014.
  17. Gadepalli, R, Kotla, S, Heckle, MR, Verma, SK, Singh, NK, Rao, GN. Novel role for p21-activated kinase 2 in thrombin-induced monocyte migration. J Biol Chem, 288 (43), 30815-31, 2013.
  18. Kotla, S, Singh, NK, Heckle, MR, Tigyi, GJ, Rao, GN. The transcription factor CREB enhances interleukin-17A production and inflammation in a mouse model of atherosclerosis. Sci Signal, 6 (293), ra83, 2013.
  19. Singh, NK, Hansen, DE, Kundumani-Sridharan, V, Rao, GN. Both Kdr and Flt1 play a vital role in hypoxia-induced Src-PLD1-PKCγ-cPLA(2) activation and retinal neovascularization. Blood, 121 (10), 1911-23, 2013.
  20. Kundumani-Sridharan V, Singh NK, Kumar S, Gadepalli R, Rao GN.. NFATc1 mediates PAK1 activation in the modulation of chemokine-induced human aortic smooth muscle cell F-actin stress fiber formation, migration, and proliferation and injury-induced vascular wall remodeling.. J Biol Chem., 30 (288), 22150-62, 2013.
  21. Gadepalli, R, Singh, NK, Kundumani-Sridharan, V, Heckle, MR, Rao, GN. Novel role of proline-rich nonreceptor tyrosine kinase 2 in vascular wall remodeling after balloon injury. Arterioscler Thromb Vasc Biol, 32 (11), 2652-61, 2012.
  22. Singh, NK, Kundumani-Sridharan, V, Kumar, S, Verma, SK, Kotla, S, Mukai, H, Heckle, MR, Rao, GN. Protein kinase N1 is a novel substrate of NFATc1-mediated cyclin D1-CDK6 activity and modulates vascular smooth muscle cell division and migration leading to inward blood vessel wall remodeling. J Biol Chem, 287 (43), 36291-304, 2012.
  23. Kundumani-Sridharan V, Van Quyen D, Subramani J, Singh NK, Chin YE, Rao GN.. Novel interactions between NFATc1 and STAT-3 mediate G protein-coupled receptor agonist, thrombin-induced biphasic expression of cyclin D1, with first phase influencing cell migration and second phase directing cell proliferation.. J Biol Chem., 27 (287), 22463-82, 2012.
  24. Singh, NK, Kundumani-Sridharan, V, Rao, GN. 12/15-Lipoxygenase gene knockout severely impairs ischemia-induced angiogenesis due to lack of Rac1 farnesylation. Blood, 118 (20), 5701-12, 2011.
  25. Singh NK, Wang D, Kundumani-Sridharan V, Van Quyen D, Niu J, Rao GN.. 15-Lipoxygenase-1-enhanced Src-Janus kinase 2-STAT3 stimulation and MCP-1 expression require redox-sensitive activation of epidermal growth factor receptor in vascular wall remodeling.. J Biol Chem., 25 (286), 22478-88, 2011.
  26. Zhang Q, Wang D, Singh NK, Kundumani-Sridharan V, Gadiparthi L, Rao ChM, Rao GN.. Activation of cytosolic phospholipase A2 downstream of the Src-phospholipase D1 (PLD1)-protein kinase C γ (PKCγ) signaling axis is required for hypoxia-induced pathological retinal angiogenesis.. J Biol Chem., 25 (286), 22489-98, 2011.
  27. Singh NK, Quyen DV, Kundumani-Sridharan V, Brooks PC, Rao GN.. AP-1 (Fra-1/c-Jun)-mediated induction of expression of matrix metalloproteinase-2 is required for 15S-hydroxyeicosatetraenoic acid-induced angiogenesis.. J Biol Chem., 22 (285), 16830-43, 2010.
  28. Kundumani-Sridharan V, Niu J, Wang D, Van Quyen D, Zhang Q, Singh NK, Subramani J, Karri S, Rao GN.. 15(S)-hydroxyeicosatetraenoic acid-induced angiogenesis requires Src-mediated Egr-1-dependent rapid induction of FGF-2 expression.. Blood, 10 (115), 2105-16, 2010.
  29. Kumar A, Singh NK, Sinha PR.. Inhibition of 1,2-dimethylhydrazine induced colon genotoxicity in rats by the administration of probiotic curd.. Mol Biol Rep., 3 (37), 1373-6, 2010.
  30. Kumar A, Singh NK, Sinha PR, Kumar R.. Intervention of Acidophilus-casei dahi and wheat bran against molecular alteration in colon carcinogenesis.. Mol Biol Rep., 1 (37), 621-7, 2010.
  31. Potula HS, Wang D, Quyen DV, Singh NK, Kundumani-Sridharan V, Karpurapu M, Park EA, Glasgow WC, Rao GN.. Src-dependent STAT-3-mediated expression of monocyte chemoattractant protein-1 is required for 15(S)-hydroxyeicosatetraenoic acid-induced vascular smooth muscle cell migration.. J Biol Chem., 45 (284), 31142-55, 2009.
  32. Karpurapu M, Wang D, Singh NK, Li Q, Rao GN.. NFATc1 targets cyclin A in the regulation of vascular smooth muscle cell multiplication during restenosis.. J Biol Chem., 39 (283), 26577-90, 2008.