LIDIA GARDNER, Ph.D.

Associate Professor
Neurology

Office: RES 151 VETERANS AFFAIRS MEDICAL CENTER
1030 JEFFERSON AVENUE
MEMPHIS TN 381042193
Tel: (901) 523-8990
lgardne5@uthsc.edu

Download My Curriculum Vitae

Research Description

My research interests span from biochemistry of G-protein coupled receptors to methionine adenosyltransferase enzyme and its role in the one carbon cycle; to the pathology and metabolomics of multiple sclerosis (MS). My recent discovery of differential Apolipoprotein A-I expression in biological fluids of MS patients and age- matched controls led me to investigate the role and turnover of lipoproteins within the nervous system.  MS is a multifactorial disease that affects immune, metabolic and nervous systems. Despite more than a century of research in this area MS remains incurable today. Research into involvement of lipoproteins into pathology of this disease offers novel insights into development of treatment options.  We utilize a mouse model of MS to investigate mechanisms of lipoproteins involvement in immune-mediated neurological disease.

Research Interest/Specialty

Multiple sclerosis and other autoimmune diseases of the central nervous system (CNS). Apolipoproteins and their turnover within the CNS. Immune-mediated neurological diseases. G-protein coupled receptors. Metabolic changes associated with autoimmune diseases.

Research Keywords

Autoimmune diseases of the central nervous system (CNS), multiple sclerosis. Role of apolipoproteins in health and disease.

Publications

  1. Levin, MC, Lee, S, Gardner, LA, Shin, Y, Douglas, JN, Cooper, C. Autoantibodies to Non-myelin Antigens as Contributors to the Pathogenesis of Multiple Sclerosis. J Clin Cell Immunol, 4, 2013.
  2. Gardner, LA, Desiderio, DM, Groover, CJ, Hartzes, A, Yates, CR, Zucker-Levin, AR, Bloom, L, Levin, MC. LC-MS/MS identification of the one-carbon cycle metabolites in human plasma. Electrophoresis, 34 (11), 1710-6, 2013.
  3. Hajjhussein, H, Gardner, LA, Fujii, N, Anderson, NM, Bahouth, SW. The hydrophobic amino acid cluster at the cytoplasmic end of transmembrane helix III modulates the coupling of the β(1)-adrenergic receptor to G(s). J Recept Signal Transduct Res, 33 (2), 79-88, 2013.
  4. Douglas, JN, Gardner, LA, Lee, S, Shin, Y, Groover, CJ, Levin, MC. Antibody transfection into neurons as a tool to study disease pathogenesis. J Vis Exp (67), 2012.
  5. Lee, S, Xu, L, Shin, Y, Gardner, L, Hartzes, A, Dohan, FC, Raine, C, Homayouni, R, Levin, MC. A potential link between autoimmunity and neurodegeneration in immune-mediated neurological disease. J Neuroimmunol, 235 (1-2), 56-69, 2011.
  6. Gardner, LA, Hajjhussein, H, Frederick-Dyer, KC, Bahouth, SW. Rab11a and its binding partners regulate the recycling of the ß1-adrenergic receptor. Cell Signal, 23 (1), 46-57, 2011.
  7. Attia, RR, Gardner, LA, Mahrous, E, Taxman, DJ, Legros, L, Rowe, S, Ting, JP, Geller, A, Kotb, M. Selective targeting of leukemic cell growth in vivo and in vitro using a gene silencing approach to diminish S-adenosylmethionine synthesis. J Biol Chem, 283 (45), 30788-95, 2008.
  8. Abdeltawab, NF, Aziz, RK, Kansal, R, Rowe, SL, Su, Y, Gardner, L, Brannen, C, Nooh, MM, Attia, RR, Abdelsamed, HA, Taylor, WL, Lu, L, Williams, RW, Kotb, M. An unbiased systems genetics approach to mapping genetic loci modulating susceptibility to severe streptococcal sepsis. PLoS Pathog, 4 (4), e1000042, 2008.
  9. Gardner, LA, Naren, AP, Bahouth, SW. Assembly of an SAP97-AKAP79-cAMP-dependent protein kinase scaffold at the type 1 PSD-95/DLG/ZO1 motif of the human beta(1)-adrenergic receptor generates a receptosome involved in receptor recycling and networking. J Biol Chem, 282 (7), 5085-99, 2007.
  10. Gardner, LA, Tavalin, SJ, Goehring, AS, Scott, JD, Bahouth, SW. AKAP79-mediated targeting of the cyclic AMP-dependent protein kinase to the beta1-adrenergic receptor promotes recycling and functional resensitization of the receptor. J Biol Chem, 281 (44), 33537-53, 2006.
  11. Zeitoun, O, Santos, NM, Gardner, LA, White, SW, Bahouth, SW. Mutagenesis within helix 6 of the human beta1-adrenergic receptor identifies Lysine324 as a residue involved in imparting the high-affinity binding state of agonists. Mol Pharmacol, 70 (3), 838-50, 2006.
  12. Delos Santos, NM, Gardner, LA, White, SW, Bahouth, SW. Characterization of the residues in helix 8 of the human beta1-adrenergic receptor that are involved in coupling the receptor to G proteins. J Biol Chem, 281 (18), 12896-907, 2006.
  13. Gardner, LA, Delos Santos, NM, Matta, SG, Whitt, MA, Bahouth, SW. Role of the cyclic AMP-dependent protein kinase in homologous resensitization of the beta1-adrenergic receptor. J Biol Chem, 279 (20), 21135-43, 2004.