KUI LI, Ph.D.

Associate Professor
Dept of Micr, Immun, & Biochem

Office: 401b Molecular Sciences Building
Tel: (901) 448-2571
kli1@uthsc.edu

Education

  • Ph.D., Beijing Medical University, China
  • M.D., Beijing Medical University, China

Research interest/specialty

Pathogenesis of hepatitis C, innate immunity to viral infections

Research interest/specialty

Pathogenesis of hepatitis C, innate immunity to viral infections

Publications

  1. Eyre, NS, Fiches, GN, Aloia, AL, Helbig, KJ, McCartney, EM, McErlean, CS, Li, K, Aggarwal, A, Turville, SG, Beard, MR. Dynamic Imaging of the Hepatitis C Virus NS5A Protein during a Productive Infection. J Virol, 88 (7), 3636-52, 2014.
  2. Lester SN, Li K. Toll-Like Receptors in Antiviral Innate Immunity. J Mol Biol, 426 (6), 1246-1264, 2014.
  3. Liu, S, Qiu, C, Miao, R, Zhou, J, Lee, A, Liu, B, Lester, SN, Fu, W, Zhu, L, Zhang, L, Xu, J, Fan, D, Li, K, Fu, M, Wang, T. MCPIP1 restricts HIV infection and is rapidly degraded in activated CD4+ T cells. Proc Natl Acad Sci U S A, 110 (47), 19083-19088, 2013.
  4. Brownell, J, Wagoner, J, Lovelace, ES, Thirstrup, D, Mohar, I, Smith, W, Giugliano, S, Li, K, Crispe, IN, Rosen, HR, Polyak, SJ. Independent, parallel pathways to CXCL10 induction in HCV-infected hepatocytes. J Hepatol, 59 (4), 701-8, 2013.
  5. Li K, Lemon SM. Innate immune responses in hepatitis C virus infection. Semin Immunopathol, 35 (1), 53-72, 2013.
  6. Orr WS, Denbo JW, Saab KR, Ng CY, Wu J, Li K, Garner JM, Morton CL, Du Z, Pfeffer LM, Davidoff AM. Curcumin Potentiates Rhabdomyosarcoma Radiosensitivity by Suppressing NF-κB Activity. PLoS ONE, 8 (2), e51309, 2013.
  7. Li G, Li K, Lea AS, Li NL, Abdulla NE, Eltorky MA, Ferguson MR. In situ hybridization for the detection of hepatitis C virus RNA in human liver tissue. J Viral Hepat, 20 (3), 183-192, 2013.
  8. Zhang S, Kodys K, Li K, Szabo G. Human Type 2 Myeloid Dendritic Cells Produce Interferon-λ and Amplify Interferon-α in Response to Hepatitis C Virus Infection. Gastroenterology, 144 (2), 414-425, 2013.
  9. Wang D, Cao L, Xu Z, Fang L, Zhong Y, Chen Q, Luo R, Chen H, Li K, Xiao S. MiR-125b Reduces Porcine Reproductive and Respiratory Syndrome Virus Replication by Negatively Regulating the NF-κB Pathway. PLoS ONE, 8 (2), e55838, 2013.
  10. Song, H, Ren, F, Li, J, Shi, S, Yan, L, Gao, F, Li, K, Zhuang, H. A laboratory-adapted HCV JFH-1 strain is sensitive to neutralization and can gradually escape under the selection pressure of neutralizing human plasma. Virus Res, 169 (1), 154-61, 2012.
  11. Guo, F, Mead, J, Aliya, N, Wang, L, Cuconati, A, Wei, L, Li, K, Block, TM, Guo, JT, Chang, J. RO 90-7501 Enhances TLR3 and RLR Agonist Induced Antiviral Response. PLoS One, 7 (10), e42583, 2012.
  12. Wang D, Fang L, Li K, Zhong H, Fan J, Ouyang C, Zhang H, Duan E, Luo R, Zhang Z, Liu X, Chen H, Xiao S. Foot-and-Mouth Disease Virus 3C Protease Cleaves NEMO To Impair Innate Immune Signaling. J Virol, 86 (17), 9311-22, 2012.
  13. Li K, Li NL, Wei D, Pfeffer SR, Fan M, Pfeffer LM. Activation of chemokine and inflammatory cytokine response in hepatitis C virus-infected hepatocytes depends on Toll-like receptor 3 sensing of hepatitis C virus double-stranded RNA intermediates. Hepatology, 55 (3), 666-75, 2012.
  14. Ren J, Wang Q, Kolli D, Prusak DJ, Tseng CT, Chen ZJ, Li K, Wood TG, Bao X. Human Metapneumovirus M2-2 Protein Inhibits Innate Cellular Signaling by Targeting MAVS. J. Virol., 86 (23), 13049-61, 2012.
  15. Shen Y, Li NL, Wang J, Liu B, Lester S, Li K. TRIM56 Is an Essential Component of the TLR3 Antiviral Signaling Pathway. J Biol Chem, 287 (43), 36404-13, 2012.
  16. Sun L, Xing Y, Chen X, Zheng Y, Yang Y, Nichols DB, Clementz MA, Banach BS, Li K, Baker SC, Chen Z. Coronavirus Papain-like Proteases Negatively Regulate Antiviral Innate Immune Response through Disruption of STING-Mediated Signaling. PLoS One, 7 (2), e30802, 2012.
  17. Wang RY, Li K. Host factors in the replication of positive-strand RNA viruses. Chang Gung Med J, 35 (2), 111-124, 2012.
  18. Helbig, KJ, Eyre, NS, Yip, E, Narayana, S, Li, K, Fiches, G, McCartney, EM, Jangra, RK, Lemon, SM, Beard, MR. The antiviral protein viperin inhibits hepatitis C virus replication via interaction with nonstructural protein 5A. Hepatology, 54 (5), 1506-17, 2011.
  19. Wilson, R, Warner, N, Ryan, K, Selleck, L, Colledge, D, Rodgers, S, Li, K, Revill, P, Locarnini, S. The hepatitis B e antigen suppresses IL-1β-mediated NF-κB activation in hepatocytes. J Viral Hepat, 18 (10), e499-507, 2011.
  20. Ren, J, Liu, T, Pang, L, Li, K, Garofalo, RP, Casola, A, Bao, X. A novel mechanism for the inhibition of interferon regulatory factor-3-dependent gene expression by human respiratory syncytial virus NS1 protein. J Gen Virol, 92 (Pt 9), 2153-9, 2011.
  21. Qu, L, Feng, Z, Yamane, D, Liang, Y, Lanford, RE, Li, K, Lemon, SM. Disruption of TLR3 Signaling Due to Cleavage of TRIF by the Hepatitis A Virus Protease-Polymerase Processing Intermediate, 3CD. PLoS Pathog, 7 (9), e1002169, 2011.
  22. Wang, J, Liu, B, Wang, N, Lee, YM, Liu, C, Li, K. TRIM56 Is a Virus- and Interferon-Inducible E3 Ubiquitin Ligase That Restricts Pestivirus Infection. J Virol, 85 (8), 3733-45, 2011.
  23. Wang, D, Fang, L, Li, P, Sun, L, Fan, J, Zhang, Q, Luo, R, Liu, X, Li, K, Chen, H, Chen, Z, Xiao, S. The Leader Proteinase of Foot-and-Mouth Disease Virus Negatively Regulates the Type I Interferon Pathway by Acting as a Viral Deubiquitinase. J Virol, 85 (8), 3758-66, 2011.
  24. Zhou, Z, Wang, N, Woodson, SE, Dong, Q, Wang, J, Liang, Y, Rijnbrand, R, Wei, L, Nichols, JE, Guo, JT, Holbrook, MR, Lemon, SM, Li, K. Antiviral activities of ISG20 in positive-strand RNA virus infections. Virology, 409 (2), 175-88, 2011.
  25. Clementz, MA, Chen, Z, Banach, BS, Wang, Y, Sun, L, Ratia, K, Baez-Santos, YM, Wang, J, Takayama, J, Ghosh, AK, Li, K, Mesecar, AD, Baker, SC. Deubiquitinating and interferon antagonism activities of coronavirus papain-like proteases. J Virol, 84 (9), 4619-29, 2010.
  26. Wang, N, Dong, Q, Li, J, Jangra, RK, Fan, M, Brasier, AR, Lemon, SM, Pfeffer, LM, Li, K. Viral induction of the zinc finger antiviral protein is IRF3-dependent but NF-kappaB-independent. J Biol Chem, 285 (9), 6080-90, 2010.
  27. Angus, AG, Dalrymple, D, Boulant, S, McGivern, DR, Clayton, RF, Scott, MJ, Adair, R, Graham, S, Owsianka, AM, Targett-Adams, P, Li, K, Wakita, T, McLauchlan, J, Lemon, SM, Patel, AH. Requirement of cellular DDX3 for hepatitis C virus replication is unrelated to its interaction with the viral core protein. J Gen Virol, 91 (Pt 1), 122-32, 2010.
  28. Song H, Li J, Shi S, Yan L, Zhuang H, Li K. Thermal stability and inactivation of hepatitis C virus grown in cell culture. Virol. J., 7 (1), 40, 2010.
  29. Liu, P, Lu, M, Tian, B, Li, K, Garofalo, RP, Prusak, D, Wood, TG, Brasier, AR. Expression of an IKKgamma splice variant determines IRF3 and canonical NF-kappaB pathway utilization in ssRNA virus infection. PLoS One, 4 (11), e8079, 2009.
  30. Li, K. Regulation of Interferon Regulatory Factor 3-Dependent Innate Immunity by the HCV NS3/4A Protease. Methods Mol Biol, 510, 211-226, 2009.
  31. Yoshikawa, T, Hill, T, Li, K, Peters, CJ, Tseng, CT. Severe acute respiratory syndrome (SARS) coronavirus-induced lung epithelial cytokines exacerbate SARS pathogenesis by modulating intrinsic functions of monocyte-derived macrophages and dendritic cells. J Virol, 83 (7), 3039-48, 2009.
  32. Kenworthy R, Lambert D, Yang F, Wang N, Chen Z, Zhu H, Zhu F, Liu C, Li K, Tang H. Short-hairpin RNAs delivered by lentiviral vector transduction trigger RIG-I-mediated IFN activation. Nucleic Acids Res., 37 (19), 6587-99, 2009.
  33. Wang N, Liang Y, Devaraj S, Wang J, Lemon SM, Li K. Toll-like receptor 3 mediates establishment of an antiviral state against hepatitis C virus in hepatoma cells. J Virol, 83 (19), 9824-34, 2009.
  34. Preiss, S, Thompson, A, Chen, X, Rodgers, S, Markovska, V, Desmond, P, Visvanathan, K, Li, K, Locarnini, S, Revill, P. Characterization of the innate immune signalling pathways in hepatocyte cell lines. J Viral Hepat, 15 (12), 888-900, 2008.
  35. Liang, Y, Ishida, H, Lenz, O, Lin, TI, Nyanguile, O, Simmen, K, Pyles, RB, Bourne, N, Yi, M, Li, K, Lemon, SM. Antiviral suppression vs restoration of RIG-I signaling by hepatitis C protease and polymerase inhibitors. Gastroenterology, 135 (5), 1710-1718.e2, 2008.
  36. Liu, P, Li, K, Garofalo, RP, Brasier, AR. Respiratory syncytial virus induces RelA release from cytoplasmic 100-kDa NF-kappa B2 complexes via a novel retinoic acid-inducible gene-I{middle dot}NF- kappa B-inducing kinase signaling pathway. J Biol Chem, 283 (34), 23169-78, 2008.
  37. Liao, S, Bao, X, Liu, T, Lai, S, Li, K, Garofalo, RP, Casola, A. Role of retinoic acid inducible gene-I in human metapneumovirus-induced cellular signalling. J Gen Virol, 89 (Pt 8), 1978-86, 2008.
  38. Xin, L, Li, K, Soong, L. Down-regulation of dendritic cell signaling pathways by Leishmania amazonensis amastigotes. Mol Immunol, 45 (12), 3371-82, 2008.
  39. Bao, X, Liu, T, Shan, Y, Li, K, Garofalo, RP, Casola, A. Human metapneumovirus glycoprotein G inhibits innate immune responses. PLoS Pathog, 4 (5), e1000077, 2008.
  40. Devaraj, SG, Wang, N, Chen, Z, Chen, Z, Tseng, M, Barretto, N, Lin, R, Peters, CJ, Tseng, CT, Baker, SC, Li, K. Regulation of IRF-3-dependent innate immunity by the papain-like protease domain of the severe acute respiratory syndrome coronavirus. J Biol Chem, 282 (44), 32208-21, 2007.
  41. Chen, Z, Rijnbrand, R, Jangra, RK, Devaraj, SG, Qu, L, Ma, Y, Lemon, SM, Li, K. Ubiquitination and proteasomal degradation of interferon regulatory factor-3 induced by Npro from a cytopathic bovine viral diarrhea virus. Virology, 366 (2), 277-92, 2007.
  42. Yang, Y, Liang, Y, Qu, L, Chen, Z, Yi, M, Li, K, Lemon, SM. Disruption of innate immunity due to mitochondrial targeting of a picornaviral protease precursor. Proc Natl Acad Sci U S A, 104 (17), 7253-8, 2007.
  43. Ishida, H, Li, K, Yi, M, Lemon, SM. p21-activated kinase 1 is activated through the mammalian target of rapamycin/p70 S6 kinase pathway and regulates the replication of hepatitis C virus in human hepatoma cells. J Biol Chem, 282 (16), 11836-48, 2007.
  44. Liu, P, Jamaluddin, M, Li, K, Garofalo, RP, Casola, A, Brasier, AR. Retinoic acid-inducible gene I mediates early antiviral response and Toll-like receptor 3 expression in respiratory syncytial virus-infected airway epithelial cells. J Virol, 81 (3), 1401-11, 2007.
  45. Chen, Z, Benureau, Y, Rijnbrand, R, Yi, J, Wang, T, Warter, L, Lanford, RE, Weinman, SA, Lemon, SM, Martin, A, Li, K. GB virus B disrupts RIG-I signaling by NS3/4A-mediated cleavage of the adaptor protein MAVS. J Virol, 81 (2), 964-76, 2007.
  46. Loo, YM, Owen, DM, Li, K, Erickson, AK, Johnson, CL, Fish, PM, Carney, DS, Wang, T, Ishida, H, Yoneyama, M, Fujita, T, Saito, T, Lee, WM, Hagedorn, CH, Lau, DT, Weinman, SA, Lemon, SM, Gale, M. Viral and therapeutic control of IFN-beta promoter stimulator 1 during hepatitis C virus infection. Proc Natl Acad Sci U S A, 103 (15), 6001-6, 2006.
  47. Hara, Y, Hino, K, Okuda, M, Furutani, T, Hidaka, I, Yamaguchi, Y, Korenaga, M, Li, K, Weinman, SA, Lemon, SM, Okita, K. Hepatitis C virus core protein inhibits deoxycholic acid-mediated apoptosis despite generating mitochondrial reactive oxygen species. J Gastroenterol, 41 (3), 257-68, 2006.
  48. Munakata, T, Nakamura, M, Liang, Y, Li, K, Lemon, SM. Down-regulation of the retinoblastoma tumor suppressor by the hepatitis C virus NS5B RNA-dependent RNA polymerase. Proc Natl Acad Sci U S A, 102 (50), 18159-64, 2005.
  49. Ferreon, JC, Ferreon, AC, Li, K, Lemon, SM. Molecular determinants of TRIF proteolysis mediated by the hepatitis C virus NS3/4A protease. J Biol Chem, 280 (21), 20483-92, 2005.
  50. Li, K, Chen, Z, Kato, N, Gale, M, Lemon, SM. Distinct poly(I-C) and virus-activated signaling pathways leading to interferon-beta production in hepatocytes. J Biol Chem, 280 (17), 16739-47, 2005.
  51. Sumpter, R, Loo, YM, Foy, E, Li, K, Yoneyama, M, Fujita, T, Lemon, SM, Gale, M. Regulating intracellular antiviral defense and permissiveness to hepatitis C virus RNA replication through a cellular RNA helicase, RIG-I. J Virol, 79 (5), 2689-99, 2005.
  52. Lemon, SM, Yi, M, Li, K. "Strong reasons make strong actions"--The antiviral efficacy of NS3/4A protease inhibitors. Hepatology, 41 (3), 671-4, 2005.
  53. Foy, E, Li, K, Sumpter, R, Loo, YM, Johnson, CL, Wang, C, Fish, PM, Yoneyama, M, Fujita, T, Lemon, SM, Gale, M. Control of antiviral defenses through hepatitis C virus disruption of retinoic acid-inducible gene-I signaling. Proc Natl Acad Sci U S A, 102 (8), 2986-91, 2005.
  54. Li, K, Foy, E, Ferreon, JC, Nakamura, M, Ferreon, AC, Ikeda, M, Ray, SC, Gale, M, Lemon, SM. Immune evasion by hepatitis C virus NS3/4A protease-mediated cleavage of the Toll-like receptor 3 adaptor protein TRIF. Proc Natl Acad Sci U S A, 102 (8), 2992-7, 2005.
  55. Locarnini, S, Shaw, T, Dean, J, Colledge, D, Thompson, A, Li, K, Lemon, SM, Lau, GG, Beard, MR. Cellular response to conditional expression of the hepatitis B virus precore and core proteins in cultured hepatoma (Huh-7) cells. J Clin Virol, 32 (2), 113-21, 2005.
  56. Otani, K, Korenaga, M, Beard, MR, Li, K, Qian, T, Showalter, LA, Singh, AK, Wang, T, Weinman, SA. Hepatitis C virus core protein, cytochrome P450 2E1, and alcohol produce combined mitochondrial injury and cytotoxicity in hepatoma cells. Gastroenterology, 128 (1), 96-107, 2005.
  57. Sun, J, Li, K, Shata, MT, Chan, TS. The immunologic basis for hepatitis C infection. Curr Opin Gastroenterol, 20 (6), 598-602, 2004.
  58. Scholle, F*, Li, K*, Bodola, F, Ikeda, M, Luxon, BA, Lemon, SM. Virus-host cell interactions during hepatitis C virus RNA replication: impact of polyprotein expression on the cellular transcriptome and cell cycle association with viral RNA synthesis. J Virol, 78 (3), 1513-24, 2004.
  59. Konan, KV, Giddings, TH, Ikeda, M, Li, K, Lemon, SM, Kirkegaard, K. Nonstructural protein precursor NS4A/B from hepatitis C virus alters function and ultrastructure of host secretory apparatus. J Virol, 77 (14), 7843-55, 2003.
  60. Foy, E, Li, K, Wang, C, Sumpter, R, Ikeda, M, Lemon, SM, Gale, M. Regulation of interferon regulatory factor-3 by the hepatitis C virus serine protease. Science, 300 (5622), 1145-8, 2003.
  61. Li, K, Zhuang, H, Zhu, W. Partial nucleotide sequencing of hepatitis E viruses detected in sera of patients with hepatitis E from 14 cities in China. Chin Med J (Engl), 115 (7), 1058-63, 2002.
  62. Li, K, Prow, T, Lemon, SM, Beard, MR. Cellular response to conditional expression of hepatitis C virus core protein in Huh7 cultured human hepatoma cells. Hepatology, 35 (5), 1237-46, 2002.
  63. Ikeda, M, Yi, M, Li, K, Lemon, SM. Selectable subgenomic and genome-length dicistronic RNAs derived from an infectious molecular clone of the HCV-N strain of hepatitis C virus replicate efficiently in cultured Huh7 cells. J Virol, 76, 2997-3006, 2002.
  64. Okuda, M*, Li, K*, Beard, MR, Showalter, LA, Scholle, F, Lemon, SM, Weinman, SA. Mitochondrial injury, oxidative stress and antioxidant gene expression are induced by hepatitis C virus core protein. Gastroenterology, 122, 366-375, 2002.
  65. Im, SW, Zhang, JZ, Zhuang, H, Che, XY, Zhu, WF, Xu, GM, Li, K, Xia, NS, Ng, MH. A bacterially expressed peptide prevents experimental infection of primates by the hepatitis E virus. Vaccine, 19 (27), 3726-32, 2001.