KAREN A. HASTY, Ph.D., M.S., B.S.

George Thomas Wilhelm Professor of Orthopaedic Surgery

Office: Be116 Veterans Affairs Medical Center
Tel: (901) 523-8990
khasty@uthsc.edu

Education

  • Ph.D., University of Tennessee Health Science Center, Memphis, TN, Anatomy
  • M.S., University of Tennessee the Health Science Center, Memphis, Anatomy
  • B.S., University of Tennessee, Knoxville, TN, Microbiology

Publications

  1. Xiong, Q, Jiao, Y, Hasty, KA, Canale, ST, Stuart, JM, Beamer, WG, Deng, HW, Baylink, D, Gu, W. Quantitative trait loci, genes, and polymorphisms that regulate bone mineral density in mouse. Genomics, 2009.
  2. Xiong, Q, Jiao, Y, Hasty, KA, Stuart, JM, Postlethwaite, A, Kang, AH, Gu, W. Genetic and molecular basis of quantitative trait loci of arthritis in rat: genes and polymorphisms. J Immunol, 181 (2), 859-64, 2008.
  3. Xiong, Q, Zhu, J, Hasty, KA, Canale, ST, Stuart, J, Gu, W. The complexity in hunting for candidate genes within QTL that determine susceptibility to arthritis in rats. Crit Rev Immunol, 28 (2), 127-57, 2008.
  4. Xing, Z, Schwab, LP, Alley, CF, Hasty, KA, Smith, RA. Titanium particles that have undergone phagocytosis by macrophages lose the ability to activate other macrophages. J Biomed Mater Res B Appl Biomater, 85 (1), 37-41, 2008.
  5. Huang, J, Ballou, LR, Hasty, KA. Cyclic equibiaxial tensile strain induces both anabolic and catabolic responses in articular chondrocytes. Gene, 404 (1-2), 101-9, 2007.
  6. Xing, Z, Hasty, KA, Smith, RA. Administration of pamidronate alters bone-titanium attachment in the presence of endotoxin-coated polyethylene particles. J Biomed Mater Res B Appl Biomater, 83 (2), 354-8, 2007.
  7. Herring, PA, Ingels, J, Palmieri, G, Hasty, KA. 1alpha,25-Dihydroxyvitamin D3 enhances proliferation of rat prostate cancer cells in the presence of living bone. J Steroid Biochem Mol Biol, 103 (3-5), 737-41, 2007.
  8. Park, K, Min, BH, Han, DK, Hasty, K. Quantitative analysis of temporal and spatial variations of chondrocyte behavior in engineered cartilage during long-term culture. Ann Biomed Eng, 35 (3), 419-28, 2007.
  9. Park, K, Hoffmeister, B, Han, DK, Hasty, K. Therapeutic ultrasound effects on interleukin-1beta stimulated cartilage construct in vitro. Ultrasound Med Biol, 33 (2), 286-95, 2007.
  10. Schwab, LP, Xing, Z, Hasty, KA, Smith, RA. Titanium particles and surface-bound LPS activate different pathways in IC-21 macrophages. J Biomed Mater Res B Appl Biomater, 79 (1), 66-73, 2006.
  11. Park, K, Huang, J, Azar, F, Jin, RL, Min, BH, Han, DK, Hasty, K. Scaffold-free, engineered porcine cartilage construct for cartilage defect repair--in vitro and in vivo study. Artif Organs, 30 (8), 586-96, 2006.
  12. Kanangat, S, Postlethwaite, A, Hasty, K, Kang, A, Smeltzer, M, Appling, W, Schaberg, D. Induction of multiple matrix metalloproteinases in human dermal and synovial fibroblasts by Staphylococcus aureus: implications in the pathogenesis of septic arthritis and other soft tissue infections. Arthritis Res Ther, 8 (6), R176, 2006.
  13. Xing, Z, Pabst, MJ, Hasty, KA, Smith, RA. Accumulation of LPS by polyethylene particles decreases bone attachment to implants. J Orthop Res, 24 (5), 959-66, 2006.
  14. Kanangat, S, Postlethwaite, AE, Higgins, GC, Hasty, KA. Novel functions of intracellular IL-1ra in human dermal fibroblasts: implications in the pathogenesis of fibrosis. J Invest Dermatol, 126 (4), 756-65, 2006.
  15. Elder, SH, Sanders, SW, McCulley, WR, Marr, ML, Shim, JW, Hasty, KA. Chondrocyte response to cyclic hydrostatic pressure in alginate versus pellet culture. J Orthop Res, 24 (4), 740-7, 2006.
  16. Ahn, JI, Erdin, RA, Smith, R, Canale, ST, Hasty, KA. Chondrocyte injection in distraction epiphysiolysis (rabbit model). J Orthop Res, 24 (3), 355-65, 2006.
  17. Wenger, KH, Woods, JA, Holecek, A, Eckstein, EC, Robertson, JT, Hasty, KA. Matrix remodeling expression in anulus cells subjected to increased compressive load. Spine, 30 (10), 1122-6, 2005.
  18. Ahn, JI, Terry Canale, S, Butler, SD, Hasty, KA. Stem cell repair of physeal cartilage. J Orthop Res, 22 (6), 1215-21, 2004.
  19. Cunningham, AC, Hasty, KA, Enghild, JJ, Mast, AE. Structural and functional characterization of tissue factor pathway inhibitor following degradation by matrix metalloproteinase-8. Biochem J, 367 (Pt 2), 451-8, 2002.
  20. Levin, MC, Lee, SM, Kalume, F, Morcos, Y, Dohan, FC, Hasty, KA, Callaway, JC, Zunt, J, Desiderio, D, Stuart, JM. Autoimmunity due to molecular mimicry as a cause of neurological disease. Nat Med, 8 (5), 509-13, 2002.
  21. Wu, CW, Tchetina, EV, Mwale, F, Hasty, K, Pidoux, I, Reiner, A, Chen, J, Van Wart, HE, Poole, AR. Proteolysis involving matrix metalloproteinase 13 (collagenase-3) is required for chondrocyte differentiation that is associated with matrix mineralization. J Bone Miner Res, 17 (4), 639-51, 2002.
  22. Shlopov, BV, Stuart, JM, Gumanovskaya, ML, Hasty, KA. Regulation of cartilage collagenase by doxycycline. J Rheumatol, 28 (4), 835-42, 2001.
  23. Siller-López, F, García-Bañuelos, J, Hasty, KA, Segura, J, Ramos-Márquez, M, Qoronfleh, MW, Aguilar-Cordova, E, Armendáriz-Borunda, J. Truncated active matrix metalloproteinase-8 gene expression in HepG2 cells is active against native type I collagen. J Hepatol, 33 (5), 758-63, 2000.
  24. Mast, AE, Stadanlick, JE, Lockett, JM, Dietzen, DJ, Hasty, KA, Hall, CL. Tissue factor pathway inhibitor binds to platelet thrombospondin-1. J Biol Chem, 275 (41), 31715-21, 2000.
  25. Sun, HB, Smith, GN, Hasty, KA, Yokota, H. Atomic force microscopy-based detection of binding and cleavage site of matrix metalloproteinase on individual type II collagen helices. Anal Biochem, 283 (2), 153-8, 2000.
  26. Shlopov, BV, Gumanovskaya, ML, Hasty, KA. Autocrine regulation of collagenase 3 (matrix metalloproteinase 13) during osteoarthritis. Arthritis Rheum, 43 (1), 195-205, 2000.
  27. Smith, GN, Mickler, EA, Hasty, KA, Brandt, KD. Specificity of inhibition of matrix metalloproteinase activity by doxycycline: relationship to structure of the enzyme. Arthritis Rheum, 42 (6), 1140-6, 1999.
  28. Shlopov, BV, Smith, GN, Cole, AA, Hasty, KA. Differential patterns of response to doxycycline and transforming growth factor beta1 in the down-regulation of collagenases in osteoarthritic and normal human chondrocytes. Arthritis Rheum, 42 (4), 719-27, 1999.
  29. Horton, WE, Udo, I, Precht, P, Balakir, R, Hasty, K. Cytokine inducible matrix metalloproteinase expression in immortalized rat chondrocytes is independent of nitric oxide stimulation. In Vitro Cell Dev Biol Anim, 34 (5), 378-84, 1998.
  30. Shlopov, BV, Hasty, KA. Collagenase expression by normal human eosinophils. J Leukoc Biol, 63 (4), 451-5, 1998.
  31. Lamoreaux, WJ, Fitzgerald, ME, Reiner, A, Hasty, KA, Charles, ST. Vascular endothelial growth factor increases release of gelatinase A and decreases release of tissue inhibitor of metalloproteinases by microvascular endothelial cells in vitro. Microvasc Res, 55 (1), 29-42, 1998.
  32. Shlopov, BV, Lie, WR, Mainardi, CL, Cole, AA, Chubinskaya, S, Hasty, KA. Osteoarthritic lesions: involvement of three different collagenases. Arthritis Rheum, 40 (11), 2065-74, 1997.
  33. Smith, GN, Brandt, KD, Mickler, EA, Hasty, KA. Inhibition of recombinant human neutrophil collagenase by doxycycline is pH dependent. J Rheumatol, 24 (9), 1769-73, 1997.
  34. Cole, AA, Chubinskaya, S, Schumacher, B, Huch, K, Szabo, G, Yao, J, Mikecz, K, Hasty, KA, Kuettner, KE. Chondrocyte matrix metalloproteinase-8. Human articular chondrocytes express neutrophil collagenase. J Biol Chem, 271 (18), 11023-6, 1996.
  35. Smith, GN, Brandt, KD, Hasty, KA. Activation of recombinant human neutrophil procollagenase in the presence of doxycycline results in fragmentation of the enzyme and loss of enzyme activity. Arthritis Rheum, 39 (2), 235-44, 1996.
  36. Chubinskaya, S, Huch, K, Mikecz, K, Cs-Szabo, G, Hasty, KA, Kuettner, KE, Cole, AA. Chondrocyte matrix metalloproteinase-8: up-regulation of neutrophil collagenase by interleukin-1 beta in human cartilage from knee and ankle joints. Lab Invest, 74 (1), 232-40, 1996.
  37. Pourmotabbed, T, Aelion, JA, Tyrrell, D, Hasty, KA, Bu, CH, Mainardi, CL. Role of the conserved histidine and aspartic acid residues in activity and stabilization of human gelatinase B: an example of matrix metalloproteinases. J Protein Chem, 14 (7), 527-35, 1995.
  38. Smith, GN, Brandt, KD, Hasty, KA. Procollagenase is reduced to inactive fragments upon activation in the presence of doxycycline. Ann N Y Acad Sci, 732, 436-8, 1994.
  39. Pourmotabbed, T, Solomon, TL, Hasty, KA, Mainardi, CL. Characteristics of 92 kDa type IV collagenase/gelatinase produced by granulocytic leukemia cells: structure, expression of cDNA in E. coli and enzymic properties. Biochim Biophys Acta, 1204 (1), 97-107, 1994.
  40. Hasty, KA, Wu, H, Byrne, M, Goldring, MB, Seyer, JM, Jaenisch, R, Krane, SM, Mainardi, CL. Susceptibility of type I collagen containing mutated alpha 1(1) chains to cleavage by human neutrophil collagenase. Matrix, 13 (3), 181-6, 1993.
  41. Hirose, T, Patterson, C, Pourmotabbed, T, Mainardi, CL, Hasty, KA. Structure-function relationship of human neutrophil collagenase: identification of regions responsible for substrate specificity and general proteinase activity. Proc Natl Acad Sci U S A, 90 (7), 2569-73, 1993.
  42. Fillmore, HL, Mainardi, CL, Hasty, KA. Differentiation of PC12 cells with nerve growth factor is associated with induction of transin synthesis and release. J Neurosci Res, 31 (4), 662-9, 1992.
  43. Terato, K, Hasty, KA, Reife, RA, Cremer, MA, Kang, AH, Stuart, JM. Induction of arthritis with monoclonal antibodies to collagen. J Immunol, 148 (7), 2103-8, 1992.
  44. Hirose, T, Reife, RA, Smith, GN, Stevens, RM, Mainardi, CL, Hasty, KA. Characterization of type V collagenase (gelatinase) in synovial fluid of patients with inflammatory arthritis. J Rheumatol, 19 (4), 593-9, 1992.
  45. Desrochers, PE, Mookhtiar, K, Van Wart, HE, Hasty, KA, Weiss, SJ. Proteolytic inactivation of alpha 1-proteinase inhibitor and alpha 1-antichymotrypsin by oxidatively activated human neutrophil metalloproteinases. J Biol Chem, 267 (7), 5005-12, 1992.
  46. Fillmore, HL, Mainardi, CL, Hasty, KA. Induction of stromelysin synthesis is associated with PC12 cell differentiation stimulated by nerve growth factor. Matrix Suppl, 1, 408, 1992.
  47. Reife, RA, Hasty, KA, Mainardi, CL, Stuart, JM. Immunolocalization of metalloproteinases in collagen autoimmune arthritis. Matrix Suppl, 1, 397, 1992.
  48. Yu, LP, Smith, GN, Hasty, KA, Brandt, KD. Doxycycline inhibits type XI collagenolytic activity of extracts from human osteoarthritic cartilage and of gelatinase. J Rheumatol, 18 (10), 1450-2, 1991.
  49. Mainardi, CL, Pourmotabbed, TF, Hasty, KA. Inflammatory phagocytes and connective tissue degrading metalloproteinases. Am J Med Sci, 302 (3), 171-5, 1991.
  50. Reife, RA, Loutis, N, Watson, WC, Hasty, KA, Stuart, JM. SWR mice are resistant to collagen-induced arthritis but produce potentially arthritogenic antibodies. Arthritis Rheum, 34 (6), 776-81, 1991.
  51. Smith, GN, Hasty, KA, Yu, LP, Lamberson, KS, Mickler, EA, Brandt, KD. Cleavage of type XI collagen fibers by gelatinase and by extracts of osteoarthritic canine cartilage. Matrix, 11 (1), 36-42, 1991.
  52. Hasty, KA, Pourmotabbed, TF, Goldberg, GI, Thompson, JP, Spinella, DG, Stevens, RM, Mainardi, CL. Human neutrophil collagenase. A distinct gene product with homology to other matrix metalloproteinases. J Biol Chem, 265 (20), 11421-4, 1990.
  53. Mainardi, CL, Hasty, KA. Secretion and glycosylation of rabbit macrophage type V collagenase. Matrix, 10 (2), 84-90, 1990.
  54. Hasty, KA, Reife, RA, Kang, AH, Stuart, JM. The role of stromelysin in the cartilage destruction that accompanies inflammatory arthritis. Arthritis Rheum, 33 (3), 388-97, 1990.
  55. Smith, GN, Hasty, KA, Brandt, KD. Type XI collagen is associated with the chondrocyte surface in suspension culture. Matrix, 9 (3), 186-92, 1989.
  56. Seyer, JM, Hasty, KA, Kang, AH. Covalent structure of collagen. Amino acid sequence of an arthritogenic cyanogen bromide peptide from type II collagen of bovine cartilage. Eur J Biochem, 181 (1), 159-73, 1989.
  57. Postlethwaite, AE, Smith, GN, Lachman, LB, Endres, RO, Poppleton, HM, Hasty, KA, Seyer, JM, Kang, AH. Stimulation of glycosaminoglycan synthesis in cultured human dermal fibroblasts by interleukin 1. Induction of hyaluronic acid synthesis by natural and recombinant interleukin 1s and synthetic interleukin 1 beta peptide 163-171. J Clin Invest, 83 (2), 629-36, 1989.
  58. Chiang, TM, Jin, A, Hasty, KA, Kang, AH. Collagen-platelet interaction: inhibition by a monoclonal antibody which binds a 90,000 dalton platelet glycoprotein. Thromb Res, 53 (2), 129-43, 1989.
  59. Fitch, JM, Birk, DE, Mentzer, A, Hasty, KA, Mainardi, C, Linsenmayer, TF. Corneal collagen fibrils: dissection with specific collagenases and monoclonal antibodies. Invest Ophthalmol Vis Sci, 29 (7), 1125-36, 1988.
  60. Hasty, KA, Jeffrey, JJ, Hibbs, MS, Welgus, HG. The collagen substrate specificity of human neutrophil collagenase. J Biol Chem, 262 (21), 10048-52, 1987.
  61. Hasty, KA, Stricklin, GP, Hibbs, MS, Mainardi, CL, Kang, AH. The immunologic relationship of human neutrophil and skin collagenases. Arthritis Rheum, 30 (6), 695-9, 1987.
  62. Mainardi, CL, Hasty, KA, Hibbs, MS. Collagen degradation by inflammatory phagocytes. J Rheumatol, 14 Spec No, 59-60, 1987.
  63. Hasty, KA, Hibbs, MS, Kang, AH, Mainardi, CL. Secreted forms of human neutrophil collagenase. J Biol Chem, 261 (12), 5645-50, 1986.
  64. Terato, K, Cremer, MA, Hasty, KA, Kang, AH, Hasty, DL, Townes, AS. Physicochemical and immunological studies of the renatured alpha 1(II) chains and isolated cyanogen bromide peptides of type II collagen. Coll Relat Res, 5 (6), 469-80, 1985.
  65. Terato, K, Hasty, KA, Cremer, MA, Stuart, JM, Townes, AS, Kang, AH. Collagen-induced arthritis in mice. Localization of an arthritogenic determinant to a fragment of the type II collagen molecule. J Exp Med, 162 (2), 637-46, 1985.
  66. Hibbs, MS, Hasty, KA, Seyer, JM, Kang, AH, Mainardi, CL. Biochemical and immunological characterization of the secreted forms of human neutrophil gelatinase. J Biol Chem, 260 (4), 2493-500, 1985.
  67. Mainardi, CL, Hibbs, MS, Hasty, KA, Seyer, JM. Purification of a type V collagen degrading metalloproteinase from rabbit alveolar macrophages. Coll Relat Res, 4 (6), 479-92, 1984.
  68. Hibbs, MS, Hasty, KA, Kang, AH, Mainardi, CL. Secretion of collagenolytic enzymes by human polymorphonuclear leukocytes. Coll Relat Res, 4 (6), 467-77, 1984.
  69. Hasty, KA, Hibbs, MS, Kang, AH, Mainardi, CL. Heterogeneity among human collagenases demonstrated by monoclonal antibody that selectively recognizes and inhibits human neutrophil collagenase. J Exp Med, 159 (5), 1455-63, 1984.
  70. Mainardi, CL, Hasty, KA, Hibbs, MS. Antibody to rabbit macrophage type V collagenase/gelatinase and its use to further characterize the enzyme. Coll Relat Res, 4 (3), 209-17, 1984.
  71. Hasty, KA, Smith, GN, Kang, AH. Histochemical identification of sulfation position in chondroitin sulfate in various cartilages. J Histochem Cytochem, 31 (12), 1367-74, 1983.
  72. Hasty, KA, Smith, GN, Kang, AH. Studies on glycosaminoglycans of regenerating rabbit ear cartilage. Dev Biol, 86 (1), 198-205, 1981.
  73. Gabbay, KH, Hasty, K, Breslow, JL, Ellison, RC, Bunn, HF, Gallop, PM. Glycosylated hemoglobins and long-term blood glucose control in diabetes mellitus. J Clin Endocrinol Metab, 44 (5), 859-64, 1977.