Steinle, Jena J. PhD

Associate Professor
Ophthalmology
 
Associate Professor
Anatomy and Neurobiology

Office: 768a 930 Madison Building
Tel: (901) 448-1910
jjsteinle@uthsc.edu

Education

  • PostDoc, Texas A&M University Health Science Center, Cardiovascular Cell Biology
  • Ph.D., University of Kansas Medical Center, Physiology/Neuroscience
  • B.S., University of Bridgeport, Biology

Research description

Our lab currently works on 2 major projects. Our first hypothesis is the diabetes results in the loss of beta-adrenergic receptor signaling and that this loss causes dysfunction of retinal biochemistry and histology in retinopathy. We are currently doing in vitro work to dissect the mechanisms involved in beta-adrenergic receptor regulation of insulin and IGF-1 receptor signaling in retinal endothelial cells and Muller cells. We are also performing pre-clinical testing of a novel eye drop therapy for the prevention of retinopathy-like changes in diabetic animal models. We perform functional assessment of the animals over an 8 month period and also assess protein changes in insulin receptor signaling and inflammation. This work will continue, as the drug has been successful in preventing diabetic-like changes in the rodent retina. The second project in the lab is to determine whether the age-related loss of sympathetic neurotransmission (specifically beta-adrenergic receptor signaling) leads to an increase in IGF-1 receptor signaling and represents the critical switch from normal aging of the retina to a disease of the retina, leading to angiogenesis and blindness. This work is funded by the NIA and investigates the cellular signaling changes in the aging retina.

Research interest/specialty

Research in our lab investigates changes in the choroid and retina in age-related macular degeneration and diabetic retinopathy. We are currently working on the effects of loss in sympathetic innervation (the fight or flight system) on the retinal pathology associated with diabetic retinopathy and choroidal pathology with macular degeneration. We use modern cellular and molecular biology techniques on cell culture and animal samples to evaulate the mechanisms for the disease processes. We are al

Research keywords

Diabetic retinopathy; sympathetic nerves; beta-adrenergic receptors; retina; pharmacology; drug discovery; translational research; pre-clinical trials; aging; apoptosis; endothelial cells; Muller cells; insulin signaling; IGF-1; IGFBPs; protein analyses; inflammation; growth factors; angiogenesis; ERG;

Area of teaching/subject

Cardiovascular biology; vision; respiratory; autonomic pharmacology

Research interest/specialty

Research in our lab investigates changes in the choroid and retina in age-related macular degeneration and diabetic retinopathy. We are currently working on the effects of loss in sympathetic innervation (the fight or flight system) on the retinal pathology associated with diabetic retinopathy and choroidal pathology with macular degeneration. We use modern cellular and molecular biology techniques on cell culture and animal samples to evaulate the mechanisms for the disease processes. We are al

Research keywords

Diabetic retinopathy; sympathetic nerves; beta-adrenergic receptors; retina; pharmacology; drug discovery; translational research; pre-clinical trials; aging; apoptosis; endothelial cells; Muller cells; insulin signaling; IGF-1; IGFBPs; protein analyses; inflammation; growth factors; angiogenesis; ERG;

Research description

Our lab currently works on 2 major projects. Our first hypothesis is the diabetes results in the loss of beta-adrenergic receptor signaling and that this loss causes dysfunction of retinal biochemistry and histology in retinopathy. We are currently doing in vitro work to dissect the mechanisms involved in beta-adrenergic receptor regulation of insulin and IGF-1 receptor signaling in retinal endothelial cells and Muller cells. We are also performing pre-clinical testing of a novel eye drop therapy for the prevention of retinopathy-like changes in diabetic animal models. We perform functional assessment of the animals over an 8 month period and also assess protein changes in insulin receptor signaling and inflammation. This work will continue, as the drug has been successful in preventing diabetic-like changes in the rodent retina. The second project in the lab is to determine whether the age-related loss of sympathetic neurotransmission (specifically beta-adrenergic receptor signaling) leads to an increase in IGF-1 receptor signaling and represents the critical switch from normal aging of the retina to a disease of the retina, leading to angiogenesis and blindness. This work is funded by the NIA and investigates the cellular signaling changes in the aging retina.

Publications

  1. Steinle, JJ, Sharma, S, Smith, CP, McFayden-Ketchum, LS. Normal Aging Involves Modulation of Specific Inflammatory Markers in the Rat Retina and Choroid. J Gerontol A Biol Sci Med Sci, 2009.
  2. Steinle, JJ, Kern, TS, Thomas, SA, McFadyen-Ketchum, LS, Smith, CP. Increased basement membrane thickness, pericyte ghosts, and loss of retinal thickness and cells in dopamine beta hydroxylase knockout mice. Exp Eye Res, 2009.
  3. Steinle, JJ, Cappocia, FC, Jiang, Y. Beta-adrenergic receptor regulation of growth factor protein levels in human choroidal endothelial cells. Growth Factors, 26 (6), 325-30, 2008.
  4. Steinle, JJ, Chin, VC, Williams, KP, Panjala, SR. Beta-adrenergic receptor stimulation modulates iNOS protein levels through p38 and ERK1/2 signaling in human retinal endothelial cells. Exp Eye Res, 87 (1), 30-4, 2008.
  5. Steinle, JJ, Sharma, S, Chin, VC. Normal aging involves altered expression of growth factors in the rat choroid. J Gerontol A Biol Sci Med Sci, 63 (2), 135-40, 2008.
  6. Smith, CP, Steinle, JJ. Changes in growth factor expression in normal aging of the rat retina. Exp Eye Res, 85 (6), 817-24, 2007.
  7. Walker, RJ, Steinle, JJ. Role of beta-adrenergic receptors in inflammatory marker expression in Müller cells. Invest Ophthalmol Vis Sci, 48 (11), 5276-81, 2007.
  8. Steinle, JJ. Sympathetic neurotransmission modulates expression of inflammatory markers in the rat retina. Exp Eye Res, 84 (1), 118-25, 2007.
  9. Smith, CP, Sharma, S, Steinle, JJ. Age-related changes in sympathetic neurotransmission in rat retina and choroid. Exp Eye Res, 84 (1), 75-81, 2007.
  10. Steinle, JJ, Lashbrook, BL. Cervical sympathectomy regulates expression of key angiogenic factors in the rat choroid. Exp Eye Res, 83 (1), 16-23, 2006.
  11. Wiley, LA, Berkowitz, BA, Steinle, JJ. Superior cervical ganglionectomy induces changes in growth factor expression in the rat retina. Invest Ophthalmol Vis Sci, 47 (1), 439-43, 2006.
  12. Banz, WJ, Davis, J, Steinle, JJ, Adler, S, Oitker, J, Winters, TA, Higginbotham, DA, Hou, Y, Henry, N, Peterson, R, Meyers, CY. (+)-Z-Bisdehydrodoisynolic acid ameliorates obesity and the metabolic syndrome in female ZDF rats. Obes Res, 13 (11), 1915-24, 2005.
  13. Lashbrook, BL, Steinle, JJ. Beta-adrenergic receptor regulation of pigment epithelial-derived factor expression in rat retina. Auton Neurosci, 121 (1-2), 33-9, 2005.
  14. Steinle, JJ, Lindsay, NL, Lashbrook, BL. Cervical sympathectomy causes photoreceptor-specific cell death in the rat retina. Auton Neurosci, 120 (1-2), 46-51, 2005.
  15. Davis, J, Iqbal, MJ, Steinle, J, Oitker, J, Higginbotham, DA, Peterson, RG, Banz, WJ. Soy protein influences the development of the metabolic syndrome in male obese ZDFxSHHF rats. Horm Metab Res, 37 (5), 316-25, 2005.
  16. Davis, J, Steinle, J, Higginbotham, DA, Oitker, J, Peterson, RG, Banz, WJ. Soy protein influences insulin sensitivity and cardiovascular risk in male lean SHHF rats. Horm Metab Res, 37 (5), 309-15, 2005.
  17. Wiley, LA, Rupp, GR, Steinle, JJ. Sympathetic innervation regulates basement membrane thickening and pericyte number in rat retina. Invest Ophthalmol Vis Sci, 46 (2), 744-8, 2005.
  18. Steinle, JJ, Zamora, DO, Rosenbaum, JT, Granger, HJ. Beta 3-adrenergic receptors mediate choroidal endothelial cell invasion, proliferation, and cell elongation. Exp Eye Res, 80 (1), 83-91, 2005.
  19. Steinle, JJ, Meininger, CJ, Chowdhury, U, Wu, G, Granger, HJ. Role of ephrin B2 in human retinal endothelial cell proliferation and migration. Cell Signal, 15 (11), 1011-7, 2003.
  20. Steinle, JJ, Smith, PG. Sensory but not parasympathetic nerves are required for ocular vascular remodeling following chronic sympathectomy in rat. Auton Neurosci, 109 (1-2), 34-41, 2003.
  21. Steinle, JJ, Granger, HJ. Nerve growth factor regulates human choroidal, but not retinal, endothelial cell migration and proliferation. Auton Neurosci, 108 (1-2), 57-62, 2003.
  22. Steinle, JJ, Booz, GW, Meininger, CJ, Day, JN, Granger, HJ. Beta 3-adrenergic receptors regulate retinal endothelial cell migration and proliferation. J Biol Chem, 278 (23), 20681-6, 2003.
  23. Steinle, JJ, Meininger, CJ, Forough, R, Wu, G, Wu, MH, Granger, HJ. Eph B4 receptor signaling mediates endothelial cell migration and proliferation via the phosphatidylinositol 3-kinase pathway. J Biol Chem, 277 (46), 43830-5, 2002.
  24. Steinle, JJ, Smith, PG. Role of adrenergic receptors in vascular remodelling of the rat choroid. Br J Pharmacol, 136 (5), 730-4, 2002.
  25. Steinle, JJ, Pierce, JD, Clancy, RL, G Smith, P. Increased ocular blood vessel numbers and sizes following chronic sympathectomy in rat. Exp Eye Res, 74 (6), 761-8, 2002.
  26. Smith, PG, Warn, JD, Steinle, JJ, Krizsan-Agbas, D, Hasan, W. Modulation of parasympathetic neuron phenotype and function by sympathetic innervation. Auton Neurosci, 96 (1), 33-42, 2002.
  27. Steinle, JJ, Smith, PG. Presynaptic muscarinic facilitation of parasympathetic neurotransmission after sympathectomy in the rat choroid. J Pharmacol Exp Ther, 294 (2), 627-32, 2000.
  28. Steinle, JJ, Krizsan-Agbas, D, Smith, PG. Regional regulation of choroidal blood flow by autonomic innervation in the rat. Am J Physiol Regul Integr Comp Physiol, 279 (1), R202-9, 2000.