Pat Ryan

Associate Professor and Assistant Chair
Microbiology, Immunology, and Biochemistry

Office: STE 1009 910 MADISON BUILDING
910 MADISON AVENUE
MEMPHIS TN 381632242
Tel: (901) 448-5535
pryan@uthsc.edu

Education

  • PostDoc, E.I. du Pont de Nemours & Co., Inc., Virology
  • Ph.D., U. of North Carolina, Chapel Hill, Microbiology
  • B.S., U. of Arkansas, Bacteriology

Publications

  1. Rue, CA, Ryan, P. Pseudorabies virus glycoprotein C attachment-proficient revertants isolated through a simple, targeted mutagenesis scheme. J Virol Methods, 151 (1), 101-6, 2008.
  2. Cuthbertson, S, LeDoux, MS, Jones, S, Jones, J, Zhou, Q, Gong, S, Ryan, P, Reiner, A. Localization of preganglionic neurons that innervate choroidal neurons of pterygopalatine ganglion. Invest Ophthalmol Vis Sci, 44 (9), 3713-24, 2003.
  3. Rue, CA, Ryan, P. A role for glycoprotein C in pseudorabies virus entry that is independent of virus attachment to heparan sulfate and which involves the actin cytoskeleton. Virology, 307 (1), 12-21, 2003.
  4. Rue, CA, Ryan, P. Characterization of pseudorabies virus glycoprotein C attachment to heparan sulfate proteoglycans. J Gen Virol, 83 (Pt 2), 301-9, 2002.
  5. LeDoux, MS, Zhou, Q, Murphy, RB, Greene, ML, Ryan, P. Parasympathetic innervation of the meibomian glands in rats. Invest Ophthalmol Vis Sci, 42 (11), 2434-41, 2001.
  6. Ryan, P, Shankly, FL. A double-strand break in a herpesvirus genome stimulates targeted homologous recombination with exogenous, cloned viral sequences. J Virol Methods, 57 (1), 95-107, 1996.
  7. Flynn, SJ, Ryan, P. The receptor-binding domain of pseudorabies virus glycoprotein gC is composed of multiple discrete units that are functionally redundant. J Virol, 70 (3), 1355-64, 1996.
  8. Ryan, P, Edwards, CO. Systematic introduction of proline in a eukaryotic signal sequence suggests asymmetry within the hydrophobic core. J Biol Chem, 270 (46), 27876-9, 1995.
  9. Flynn, SJ, Ryan, P. A heterologous heparin-binding domain can promote functional attachment of a pseudorabies virus gC mutant to cell surfaces. J Virol, 69 (2), 834-9, 1995.
  10. Tomilo, M, Wilkinson, KS, Ryan, P. Can a signal sequence become too hydrophobic. J Biol Chem, 269 (50), 32016-21, 1994.
  11. Powers, L, Ryan, P. Spontaneous fusions to prv43 can suppress the export defect of pseudorabies virus gIII signal peptide mutants. J Virol, 68 (5), 2787-94, 1994.
  12. Powers, L, Wilkinson, KS, Ryan, P. Characterization of the prv43 gene of pseudorabies virus and demonstration that it is not required for virus growth in cell culture. Virology, 199 (1), 81-8, 1994.
  13. Flynn, SJ, Burgett, BL, Stein, DS, Wilkinson, KS, Ryan, P. The amino-terminal one-third of pseudorabies virus glycoprotein gIII contains a functional attachment domain, but this domain is not required for the efficient penetration of Vero cells. J Virol, 67 (5), 2646-54, 1993.
  14. Ryan, P, Robbins, A, Whealy, M, Enquist, LW. Overall signal sequence hydrophobicity determines the in vivo translocation efficiency of a herpesvirus glycoprotein. Virus Genes, 7 (1), 5-21, 1993.
  15. Ryan, JP, Whealy, ME, Robbins, AK, Enquist, LW. Analysis of pseudorabies virus glycoprotein gIII localization and modification by using novel infectious viral mutants carrying unique EcoRI sites. J Virol, 61 (10), 2962-72, 1987.
  16. Fikes, JD, Bankaitis, VA, Ryan, JP, Bassford, PJ. Mutational alterations affecting the export competence of a truncated but fully functional maltose-binding protein signal peptide. J Bacteriol, 169 (6), 2345-51, 1987.
  17. Cover, WH, Ryan, JP, Bassford, PJ, Walsh, KA, Bollinger, J, Randall, LL. Suppression of a signal sequence mutation by an amino acid substitution in the mature portion of the maltose-binding protein. J Bacteriol, 169 (5), 1794-800, 1987.
  18. Ryan, JP, Duncan, MC, Bankaitis, VA, Bassford, PJ. Intragenic reversion mutations that improve export of maltose-binding protein in Escherichia coli malE signal sequence mutants. J Biol Chem, 261 (7), 3389-95, 1986.