Professor and Director
Bioscience Research

MEMPHIS TN 381033403
Tel: (901) 448-1490


  • PostDoc, UC San Diego, Mucosal Immunology
  • D.Sc., Boston University, Oral Biology
  • M.S.D., Boston University, Endodontics
  • Certificate, Boston University, Endodontics
  • D.D.S., Taipei Medical College, Dentistry

Curriculum Vitae

Research Keywords

Stem cell biology, regeneration

Research Description

Research Interest/Specialty

I have begun working on dental stem cells in early 2000. After many years of pioneering the new field – regenerative endodontics, our team was the first to show that dental stem cells can be utilized to regenerate dental pulp and dentin in an emptied root canal space in vivo (2009). My team was also the first to show that dental stem cells can be easily reprogrammed into induced pluripotent stem (iPS) cells (2010). We also investigated the molecular biology of dental stem cells in the context of their applications for pulp regeneration evidenced in more recent publications, including their stemness and differentiation into odontoblast lineages. We have published a number of highly cited review papers retrospectively outlined the important progress in the field of pulp regeneration and prospectively discussed what remains to be investigated in order to launch stem cell-based pulp regeneration in humans. Furthermore, besides establishing a tooth fragment model using SCID mice to study pulp regeneration which has since been used by many researchers in the field, we also established a large animal model using mini-swine to investigate pulp and dentin tissue regeneration. In terms of publications, I have published (+ in press) close to 70 peer reviewed articles and 17 book chapters as well as co-edited with Dr. Irma Thesleff a ground breaking textbook “Stem cells in craniofacial development and regeneration” Wiley & Blackwell, 2013.


  1. Jamal, M, Lewandowski, SL, Lawton, ML, Huang, GT, Ikonomou, L. Derivation and characterization of putative craniofacial mesenchymal progenitor cells from human induced pluripotent stem cells. Stem Cell Res, 33, 100-109, 2018.
  2. Huang, GTJ, Garcia-Godoy, F. What is Missing for Stem Cell-Based Therapies in Dentistry. J Tenn Dent Assoc, 96 (2), 9-12, 2018.
  3. Li, D, Zou, XY, El-Ayachi, I, Romero, LO, Yu, Z, Iglesias-Linares, A, Cordero-Morales, JF, Huang, GT. Human Dental Pulp Stem Cells and Gingival Mesenchymal Stem Cells Display Action Potential Capacity In Vitro after Neuronogenic Differentiation. Stem Cell Rev, 2018.
  4. Zhu, X, Liu, J, Yu, Z, Chen, CA, Aksel, H, Azim, AA, Huang, GT. A Miniature Swine Model for Stem Cell-Based De Novo Regeneration of Dental Pulp and Dentin-Like Tissue. Tissue Eng Part C Methods, 2018.
  5. El Ayachi, I, Zhang, J, Zou, XY, Yu, Z, Wei, W, O'Connell, KMS, Huang, GT. Human dental stem cell derived transgene-free iPSCs generate functional neurons via embryoid body-mediated and direct induction methods. J Tissue Eng Regen Med, 2017.
  6. Azim, AA, Aksel, H, Margaret Jefferson, M, Huang, GT. Comparison of sodium hypochlorite extrusion by five irrigation systems using an artificial root socket model and a quantitative chemical method. Clin Oral Investig, 2017.
  7. Aksel, H, Huang, GT. Combined Effects of Vascular Endothelial Growth Factor and Bone Morphogenetic Protein 2 on Odonto/Osteogenic Differentiation of Human Dental Pulp Stem Cells In Vitro. J Endod, 43 (6), 930-935, 2017.
  8. Aksel, H, Huang, GT. Human and Swine Dental Pulp Stem Cells Form a Vascularlike Network after Angiogenic Differentiation in Comparison with Endothelial Cells: A Quantitative Analysis. J Endod, 2017.
  9. Bakopoulou, A, Huang, GT, Rouabhia, M, Geurtsen, W, About, I. Advances and New Technologies towards Clinical Application of Oral Stem Cells and Their Secretome. Stem Cells Int, 2017, 6367375, 2016.
  10. Gauthier, P, Yu, Z, Tran, QT, Bhatti, FU, Zhu, X, Huang, GT. Cementogenic genes in human periodontal ligament stem cells are downregulated in response to osteogenic stimulation while upregulated by vitamin C treatment. Cell Tissue Res, 2016.
  11. Chen, X, Liu, JY, Yue, L, Huang, GT, Zou, XY. Phosphatidylinositol 3-Kinase and Protein Kinase C Signaling Pathways Are Involved in Stromal Cell-derived Factor-1α-mediated Transmigration of Stem Cells from Apical Papilla. J Endod, 42 (7), 1076-81, 2016.
  12. Jaha, H, Husein, D, Ohyama, Y, Xu, D, Suzuki, S, Huang, GT, Mochida, Y. N-terminal Dentin Sialoprotein fragment induces type I collagen production and upregulates dentinogenesis marker expression in osteoblasts. Biochem Biophys Rep, 6, 190-196, 2016.
  13. Azim, AA, Aksel, H, Zhuang, T, Mashtare, T, Babu, JP, Huang, GT. Efficacy of 4 Irrigation Protocols in Killing Bacteria Colonized in Dentinal Tubules Examined by a Novel Confocal Laser Scanning Microscope Analysis. J Endod, 42 (6), 928-34, 2016.
  14. Homayounfar, N, Verma, P, Nosrat, A, El Ayachi, I, Yu, Z, Romberg, E, Huang, GT, Fouad, AF. Isolation, Characterization, and Differentiation of Dental Pulp Stem Cells in Ferrets. J Endod, 42 (3), 418-24, 2016.
  15. Azim, AA, Griggs, JA, Huang, GT. The Tennessee study: factors affecting treatment outcome and healing time following nonsurgical root canal treatment. Int Endod J, 49 (1), 6-16, 2016.
  16. Saoud, TM, Huang, GT, Gibbs, JL, Sigurdsson, A, Lin, LM. Management of Teeth with Persistent Apical Periodontitis after Root Canal Treatment Using Regenerative Endodontic Therapy. J Endod, 41 (10), 1743-8, 2015.
  17. Liu, JY, Chen, X, Yue, L, Huang, GT, Zou, XY. CXC Chemokine Receptor 4 Is Expressed Paravascularly in Apical Papilla and Coordinates with Stromal Cell-derived Factor-1α during Transmigration of Stem Cells from Apical Papilla. J Endod, 41 (9), 1430-6, 2015.
  18. Jamal, M, Chogle, SM, Karam, SM, Huang, GT. NOTCH3 is expressed in human apical papilla and in subpopulations of stem cells isolated from the tissue. Genes Dis, 2 (3), 261-267, 2015.
  19. Yu, Z, Gauthier, P, Tran, QT, El-Ayachi, I, Bhatti, FU, Bahabri, R, Al-Habib, M, Huang, GT. Differential Properties of Human ALP(+) Periodontal Ligament Stem Cells vs Their ALP(-) Counterparts. J Stem Cell Res Ther, 5 (7), 2015.
  20. Zhu, X, Wang, Y, Liu, Y, Huang, GT, Zhang, C. Immunohistochemical and Histochemical Analysis of Newly Formed Tissues in Root Canal Space Transplanted with Dental Pulp Stem Cells Plus Platelet-rich Plasma. J Endod, 40 (10), 1573-8, 2014.
  21. Azim, AA, Azim, KA, Deutsch, AS, Huang, GT. Acquisition of anatomic parameters concerning molar pulp chamber landmarks using cone-beam computed tomography. J Endod, 40 (9), 1298-302, 2014.
  22. Azim, AA, Lloyd, A, Huang, GT. Management of longstanding furcation perforation using a novel approach. J Endod, 40 (8), 1255-9, 2014.
  23. Lin, LM, Ricucci, D, Huang, GT. Regeneration of the dentine-pulp complex with revitalization/revascularization therapy: challenges and hopes. Int Endod J, 47 (8), 713-24, 2014.
  24. Al Shahrani, M, DiVito, E, Hughes, CV, Nathanson, D, Huang, GT. Enhanced removal of Enterococcus faecalis biofilms in the root canal using sodium hypochlorite plus photon-induced photoacoustic streaming: an in vitro study. Photomed Laser Surg, 32 (5), 260-6, 2014.
  25. Huang, GT, Garcia-Godoy, F. Missing Concepts in De Novo Pulp Regeneration. J Dent Res, 93 (8), 717-724, 2014.
  26. Zhu, W, Zhu, X, Huang, GT, Cheung, GS, Dissanayaka, WL, Zhang, C. Regeneration of dental pulp tissue in immature teeth with apical periodontitis using platelet-rich plasma and dental pulp cells. Int Endod J, 46 (10), 962-70, 2013.
  27. Al-Habib, M, Yu, Z, Huang, GT. Small molecules affect human dental pulp stem cell properties via multiple signaling pathways. Stem Cells Dev, 22 (17), 2402-13, 2013.