DAVID BRAND, Ph.D., B.S.

Associate Professor
Department of Medicine
Division of Rheumatology
Adjunct Associate Professor
Department of Microbiology, Immunology and Biochemistry

Office: Be-135 Veterans Affairs Medical Center
Tel: (901) 577-7282
dbrand@uthsc.edu
http://www.uthsc.edu/ctr/rdrcc_collagen/

Education

  • PostDoc, UTHSC, Memphis, TN, Immunology
  • Ph.D., Georgetown University, Washington, DC, Biology
  • B.S., College of William and Mary, Williamsburg, VA, Biology

Research interest/specialty

Structure/function relationships in autoimmunity, immunomodulation of T cell determinants in collagen-induced arthritis, tolerance and regulatory T cell biology
We use extensive cell culture, flow cytometry, high-speed cell sorting and other molecular techniques to help us approach our studies in regulatory T cell biology.

Research keywords

Collagen Autoimmunity Regulatory T cell Treg Arthritis Biology Mouse Model Antibody Cytokine Inflammation Extracellular Matrix ECM

Research interest/specialty

Structure/function relationships in autoimmunity, immunomodulation of T cell determinants in collagen-induced arthritis, tolerance and regulatory T cell biology We use extensive cell culture, flow cytometry, high-speed cell sorting and other molecular techniques to help us approach our studies in regulatory T cell biology.

Research keywords

Collagen Autoimmunity Regulatory T cell Treg Arthritis Biology Mouse Model Antibody Cytokine Inflammation Extracellular Matrix ECM

Research description

My research involves understanding of the relationships that exist between cells in the immune system and how they can act together to protect us from autoimmunity. Although the regulatory T cell has moved to the forefront of tolerance biology, it still holds many secrets as to how it functions and how it may help us to not only treat people with autoimmune disease, but also how it might help us to prevent the development of autoimmunity in the first place.

Publications

  1. Hutamekalin, P, Saito, T, Yamaki, K, Mizutani, N, Brand, DD, Waritani, T, Terato, K, Yoshino, S. Collagen antibody-induced arthritis in mice: development of a new arthritogenic 5-clone cocktail of monoclonal anti-type II collagen antibodies. J Immunol Methods, 343 (1), 49-55, 2009.
  2. Iwata, T, Philipovskiy, A, Fisher, AJ, Presson, RG, Chiyo, M, Lee, J, Mickler, E, Smith, GN, Petrache, I, Brand, DB, Burlingham, WJ, Gopalakrishnan, B, Greenspan, DS, Christie, JD, Wilkes, DS. Anti-type V collagen humoral immunity in lung transplant primary graft dysfunction. J Immunol, 181 (8), 5738-47, 2008.
  3. Chiyo, M, Iwata, T, Webb, TJ, Vasko, MR, Thompson, EL, Heidler, KM, Cummings, OW, Yoshida, S, Fujisawa, T, Brand, DD, Wilkes, DS. Silencing S1P1 receptors regulates collagen-V reactive lymphocyte-mediated immunobiology in the transplanted lung. Am J Transplant, 8 (3), 537-46, 2008.
  4. Iwata, T, Chiyo, M, Yoshida, S, Smith, GN, Mickler, EA, Presson, R, Fisher, AJ, Brand, DD, Cummings, OW, Wilkes, DS. Lung transplant ischemia reperfusion injury: metalloprotease inhibition down-regulates exposure of type V collagen, growth-related oncogene-induced neutrophil chemotaxis, and tumor necrosis factor-alpha expression. Transplantation, 85 (3), 417-26, 2008.
  5. Bobadilla, J. L. Love, R. B. Jankowska-Gan, E. Xu, Q. Haynes, L. D. Braun, R. K. Hayney, M. S. Munoz del Rio, A. Meyer, K. Greenspan, D. S. Torrealba, J. Heidler, K. M. Cummings, O. W. Iwata, T. Brand, D. Presson, R. Burlingham, W. J. Wilkes, D. S.. Th-17, monokines, collagen type V, and primary graft dysfunction in lung transplantation. Am J Respir Crit Care Med, 177 (6), 660-8, 2008.
  6. Burlingham, WJ, Love, RB, Jankowska-Gan, E, Haynes, LD, Xu, Q, Bobadilla, JL, Meyer, KC, Hayney, MS, Braun, RK, Greenspan, DS, Gopalakrishnan, B, Cai, J, Brand, DD, Yoshida, S, Cummings, OW, Wilkes, DS. IL-17-dependent cellular immunity to collagen type V predisposes to obliterative bronchiolitis in human lung transplants. J Clin Invest, 117 (11), 3498-506, 2007.
  7. Barnes, CP, Pemble, CW, Brand, DD, Simpson, DG, Bowlin, GL. Cross-linking electrospun type II collagen tissue engineering scaffolds with carbodiimide in ethanol. Tissue Eng, 13 (7), 1593-605, 2007.
  8. Brand, DD, Latham, KA, Rosloniec, EF. Collagen-induced arthritis. Nat Protoc, 2 (5), 1269-75, 2007.
  9. Yoshida, S, Iwata, T, Chiyo, M, Smith, GN, Foresman, BH, Mickler, EA, Heidler, KM, Cummings, OW, Fujisawa, T, Brand, DD, Baker, A, Wilkes, DS. Metalloproteinase inhibition has differential effects on alloimmunity, autoimmunity, and histopathology in the transplanted lung. Transplantation, 83 (6), 799-808, 2007.
  10. Srinivasan, M, Eri, R, Zunt, SL, Summerlin, DJ, Brand, DD, Blum, JS. Suppression of immune responses in collagen-induced arthritis by a rationally designed CD80-binding peptide agent. Arthritis Rheum, 56 (2), 498-508, 2007.
  11. Sakurai, Y, Brand, DD, Tang, B, Rosloniec, EF, Stuart, JM, Kang, AH, Myers, LK. Analog peptides of type II collagen can suppress arthritis in HLA-DR4 (DRB1*0401) transgenic mice. Arthritis Res Ther, 8 (5), R150, 2006.
  12. Yoshida, S, Haque, A, Mizobuchi, T, Iwata, T, Chiyo, M, Webb, TJ, Baldridge, LA, Heidler, KM, Cummings, OW, Fujisawa, T, Blum, JS, Brand, DD, Wilkes, DS. Anti-type V collagen lymphocytes that express IL-17 and IL-23 induce rejection pathology in fresh and well-healed lung transplants. Am J Transplant, 6 (4), 724-35, 2006.
  13. Brand, DD. Rodent models of rheumatoid arthritis. Comp Med, 55 (2), 114-22, 2005.
  14. Srinivasan, M, Lu, D, Eri, R, Brand, DD, Haque, A, Blum, JS. CD80 binding polyproline helical peptide inhibits T cell activation. J Biol Chem, 280 (11), 10149-55, 2005.
  15. Tang, B, Brand, DD, Ma, Z, Stuart, JM, Myers, LK, Kang, AH. Pathogenesis of collagen-induced arthritis: modulation of disease by arthritogenic T-cell epitope location. Immunology, 113 (3), 384-91, 2004.
  16. Myers, LK, Tang, B, Brand, DD, Rosloniec, EF, Stuart, JM, Kang, AH. Efficacy of modified recombinant type II collagen in modulating autoimmune arthritis. Arthritis Rheum, 50 (9), 3004-11, 2004.
  17. Brand, DD, Kang, AH, Rosloniec, EF. The mouse model of collagen-induced arthritis. Methods Mol Med, 102, 295-312, 2004.
  18. Cremer, MA, Ye, XJ, Myers, LK, Brand, DD, Rosloniec, EF, Kang, AH. T cell immunity to type II collagen in the biobreeding rat: the identification and characterization of RT1u-restricted T cell epitopes on alpha 1(II). J Immunol, 173 (3), 1795-801, 2004.
  19. Van den Steen, PE, Proost, P, Brand, DD, Kang, AH, Van Damme, J, Opdenakker, G. Generation of glycosylated remnant epitopes from human collagen type II by gelatinase B. Biochemistry, 43 (33), 10809-16, 2004.
  20. Myers, LK, Myllyharju, J, Nokelainen, M, Brand, DD, Cremer, MA, Stuart, JM, Bodo, M, Kivirikko, KI, Kang, AH. Relevance of posttranslational modifications for the arthritogenicity of type II collagen. J Immunol, 172 (5), 2970-5, 2004.
  21. Brand, DD, Kang, AH, Rosloniec, EF. Immunopathogenesis of collagen arthritis. Springer Semin Immunopathol, 25 (1), 3-18, 2003.
  22. Myers, LK, Pihlajamaa, T, Brand, DD, Cremer, MA, Bodo, M, Ala-Kokko, L, Kang, AH. Immunogenicity of recombinant type IX collagen in murine collagen-induced arthritis. Arthritis Rheum, 46 (4), 1086-93, 2002.
  23. Brand, DD, Whittington, KB, Rosloniec, EF. I-Aq and I-Ap bind and present similar antigenic peptides despite differing in their ability to mediate susceptibility to autoimmune arthritis. Autoimmunity, 34 (2), 133-45, 2002.
  24. Van den Steen, PE, Proost, P, Grillet, B, Brand, DD, Kang, AH, Van Damme, J, Opdenakker, G. Cleavage of denatured natural collagen type II by neutrophil gelatinase B reveals enzyme specificity, post-translational modifications in the substrate, and the formation of remnant epitopes in rheumatoid arthritis. FASEB J, 16 (3), 379-89, 2002.
  25. Brand, DD, Myers, LK, Whittington, KB, Latham, KA, Stuart, JM, Kang, AH, Rosloniec, EF. Detection of early changes in autoimmune T cell phenotype and function following intravenous administration of type II collagen in a TCR-transgenic model. J Immunol, 168 (1), 490-8, 2002.
  26. Tang, B, Brand, DD, Chiang, TM, Stuart, JM, Kang, AH, Myers, LK. Immunogenicity and arthritogenicity of recombinant CB10 in B10.RIII mice. J Immunol, 164 (1), 481-7, 2000.
  27. Tang, B, Chiang, TM, Brand, DD, Gumanovskaya, ML, Stuart, JM, Kang, AH, Myers, LK. Molecular definition and characterization of recombinant bovine CB8 and CB10: immunogenicity and arthritogenicity. Clin Immunol, 92 (3), 256-64, 1999.
  28. Myers, LK, Brand, DD, Ye, XJ, Cremer, MA, Rosloniec, EF, Bodo, M, Myllyharju, J, Helaakoski, T, Nokelainen, M, Pihlajaniemi, T, Kivirikko, K, Yang, CL, Ala-Kokko, L, Prockop, DJ, Notbohm, H, Fietzek, P, Stuart, JM, Kang, AH. Characterization of recombinant type II collagen: arthritogenicity and tolerogenicity in DBA/1 mice. Immunology, 95 (4), 631-9, 1998.
  29. Rosloniec, EF, Brand, DD, Myers, LK, Esaki, Y, Whittington, KB, Zaller, DM, Woods, A, Stuart, JM, Kang, AH. Induction of autoimmune arthritis in HLA-DR4 (DRB1*0401) transgenic mice by immunization with human and bovine type II collagen. J Immunol, 160 (6), 2573-8, 1998.
  30. Rosloniec, EF, Brand, DD, Myers, LK, Whittington, KB, Gumanovskaya, M, Zaller, DM, Woods, A, Altmann, DM, Stuart, JM, Kang, AH. An HLA-DR1 transgene confers susceptibility to collagen-induced arthritis elicited with human type II collagen. J Exp Med, 185 (6), 1113-22, 1997.
  31. Brand, DD, Marion, TN, Myers, LK, Rosloniec, EF, Watson, WC, Stuart, JM, Kang, AH. Autoantibodies to murine type II collagen in collagen-induced arthritis: a comparison of susceptible and nonsusceptible strains. J Immunol, 157 (11), 5178-84, 1996.
  32. Rosloniec, EF, Whittington, KB, Brand, DD, Myers, LK, Stuart, JM. Identification of MHC class II and TCR binding residues in the type II collagen immunodominant determinant mediating collagen-induced arthritis. Cell Immunol, 172 (1), 21-8, 1996.
  33. Rosloniec, EF, Brand, DD, Whittington, KB, Stuart, JM, Ciubotaru, M, Ward, ES. Vaccination with a recombinant V alpha domain of a TCR prevents the development of collagen-induced arthritis. J Immunol, 155 (9), 4504-11, 1995.
  34. Miyahara, H, Myers, LK, Rosloniec, EF, Brand, DD, Seyer, JM, Stuart, JM, Kang, AH. Identification and characterization of a major tolerogenic T-cell epitope of type II collagen that suppresses arthritis in B10.RIII mice. Immunology, 86 (1), 110-5, 1995.
  35. Brand, DD, Myers, LK, Terato, K, Whittington, KB, Stuart, JM, Kang, AH, Rosloniec, EF. Characterization of the T cell determinants in the induction of autoimmune arthritis by bovine alpha 1(II)-CB11 in H-2q mice. J Immunol, 152 (6), 3088-97, 1994.